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IQSEC1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153501

ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
IQSEC1, Polyclonal Antibody
Full Product Name
IQSEC1 Antibody
Product Gene Name
Product Synonym Names
IQ motif and Sec7 domain 1; ADP-ribosylation factors guanine nucleotide-exchange protein 100; ARFGEP100; BRAG2; Brefeldin-resistant Arf-GEF 2 protein; IQ motif and Sec7 domain 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
IQSEC1 antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.
Preparation and Storage
IQSEC1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
IQSEC1 peptide (MBS154105) is used for blocking the activity of IQSEC1 antibody (MBS153501)
Molecular Weight
Predicted: 106 kDa

Observed: 103 kDa
OMIM
610166
3D Structure
ModBase 3D Structure for Q6DN90
Conjugate
Unconjugated
Immunogen
IQSEC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human IQSEC1
Buffer
IQSEC1 antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-IQSEC1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-IQSEC1 antibody
IQSEC1 antibody can be used for detection of IQSEC1 by Western blot at 1 - 2 mug/ml.

Western Blot (WB) of anti-IQSEC1 antibody

Western blot analysis of IQSEC1 in A20 cell lysate with IQSEC1 antibody at 1 μg/ml.
Rabbit Anti-IQSEC1 Polyclonal Antibody – Cat# MBS153501

Immunocytochemistry (IHC) of anti-IQSEC1 antibody

Immunocytochemistry of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.
Rabbit Anti-IQSEC1 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS153501

Immunofluorescence (IF) of anti-IQSEC1 antibody

Immunofluorescence of IQSEC1 in A-20 cells with IQSEC1 antibody at 2 μg/mL.

Red: IQSEC1 Antibody (8009)
Blue: DAPI staining
Rabbit Anti-IQSEC1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153501

Other Notes

Small volumes of anti-IQSEC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IQSEC1 purchase

MBS153501 is an antibody targeting IQSEC1. Also known as IQSEC1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IQSEC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
IQ motif and SEC7 domain-containing protein 1 isoform b
NCBI Official Synonym Full Names
IQ motif and Sec7 domain 1
NCBI Official Symbol
IQSEC1
NCBI Official Synonym Symbols
BRAG2; GEP100; ARFGEP100; ARF-GEP100
UniProt Protein Name
IQ motif and SEC7 domain-containing protein 1
UniProt Synonym Protein Names
ADP-ribosylation factors guanine nucleotide-exchange protein 100; ADP-ribosylation factors guanine nucleotide-exchange protein 2; Brefeldin-resistant Arf-GEF 2 protein
UniProt Gene Name
IQSEC1
UniProt Synonym Gene Names
ARFGEP100; BRAG2; KIAA0763
UniProt Entry Name
IQEC1_HUMAN
NCBI Protein Information
IQ motif and SEC7 domain-containing protein 1; brefeldin A-resistant ARF-GEF2; brefeldin-resistant Arf-GEF 2 protein; ADP-ribosylation factors guanine nucleotide-exchange protein 2; ADP-ribosylation factors guanine nucleotide-exchange protein 100

UniProt Comments for IQSEC1

IQSEC1: In addition to accelerate GTP gamma S binding by ARFs of all three classes, it appears to function preferentially as a guanine nucleotide exchange protein for ARF6, mediating internalisation of beta-1 integrin. Belongs to the BRAG family. Interacts with ARF6. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; GAPs; GAPs, ARF

Chromosomal Location of Human Ortholog: 3p25.2

Cellular Component: membrane; trans-Golgi network; nucleus

Molecular Function: ARF guanyl-nucleotide exchange factor activity

Biological Process: vesicle-mediated transport; regulation of ARF protein signal transduction; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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