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HES5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153653

MBS153653 is an antibody targeting HES5. Also known as HES5 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HES5, Polyclonal Antibody
Full Product Name
HES5 Antibody
Product Gene Name
anti-HES5 antibody
Product Synonym Names
Hairy and enhancer of split 5; hes family bHLH transcription factor 5; bHLHb38 ; hes family bHLH transcription factor
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid; HES5 antibody is supplied in PBS containing 0.02% sodium azide.
Purity/Purification
HES5 antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.
Preparation and Storage
HES5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
HES5 peptide (MBS153939) is used for blocking the activity of HES5 antibody (MBS153653)
Molecular Weight
Predicted: 18 kDa

Observed: 17 kDa
OMIM
607348
3D Structure
ModBase 3D Structure for Q5TA89
Conjugate
Unconjugated
Immunogen
HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.
The immunogen is located within the first 50 amino acids of HESS.
Applications Tested/Suitable for anti-HES5 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-HES5 antibody
Optimal dilutions for each application to be determined by the researcher.
The information provided is a guidline for product use. This product is for research use only.

Western Blot (WB) of anti-HES5 antibody

Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μg/ml (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-HES5 Polyclonal Antibody – Cat# MBS153653

Immunohistochemistry (IHC) of anti-HES5 antibody

Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 μg/ml.
Rabbit Anti-HES5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153653

Immunofluorescence (IF) of anti-HES5 antibody

Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μg/ml.
Rabbit Anti-HES5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153653

Immunohistochemistry (IHC) of anti-HES5 antibody

Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 μg/mL.
Rabbit Anti-HES5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153653

Immunofluorescence (IF) of anti-HES5 antibody

Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 μg/mL.

Green: HES5 Antibody (8055)
Blue: DAPI staining
Rabbit Anti-HES5 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153653

Other Notes

Small volumes of anti-HES5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HES5. It may not necessarily be applicable to this product.

NCBI GI #
58219048
NCBI GeneID
388585
NCBI Accession #
NP_001010926
UniProt Primary Accession #
Q5TA89
UniProt Secondary Accession #
B9DI85
UniProt Related Accession #
Q5TA89
GenBank Nucleotide #
NM_001010926.3
NCBI Official Full Name
transcription factor HES-5
NCBI Official Synonym Full Names
hes family bHLH transcription factor 5
NCBI Official Symbol
HES5
NCBI Official Synonym Symbols
bHLHb38
UniProt Protein Name
Transcription factor HES-5
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 38; bHLHb38; Hairy and enhancer of split 5
Protein Family
Transcription factor
UniProt Gene Name
HES5
UniProt Synonym Gene Names
BHLHB38; bHLHb38
UniProt Entry Name
HES5_HUMAN
NCBI Protein Information
transcription factor HES-5; hairy and enhancer of split 5; class B basic helix-loop-helix protein 38

NCBI Summary for HES5

This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008]
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UniProt Comments for HES5

HES5: Transcriptional repressor of genes that require a bHLH protein for their transcription.

Protein type: Transcription regulation; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1p36.32

Cellular Component: nucleoplasm

Molecular Function: protein dimerization activity; double-stranded DNA binding; chromatin binding; transcription factor binding

Biological Process: neural tube development; radial glial cell differentiation in the forebrain; regulation of myelination; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; cell maturation; positive regulation of JAK-STAT cascade; auditory receptor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of neurogenesis; astrocyte differentiation; regulation of cell differentiation; positive regulation of cell proliferation; chondrocyte differentiation; protein complex assembly; cell adhesion; oligodendrocyte development; positive regulation of BMP signaling pathway; positive regulation of Notch signaling pathway; smoothened signaling pathway; Notch signaling pathway; camera-type eye development; auditory receptor cell fate determination; transcription, DNA-dependent; myelination in the central nervous system; negative regulation of oligodendrocyte differentiation; negative regulation of neuron differentiation; telencephalon development; glial cell fate commitment; cartilage development; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; brain development
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Research Articles on HES5

1. the downstream Notch signalling effector HES5 directly represses transcription of the E3 ligase Fbw7beta.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HES5 antibody

 Reference Product  PubMed Publications
 NOTCH1 antibody  >99 publications with HES5 and NOTCH1
 DLL1 antibody  >25 publications with HES5 and DLL1
 JAG1 antibody  >19 publications with HES5 and JAG1
 NOTCH3 antibody  >18 publications with HES5 and NOTCH3
 JAG2 antibody  >12 publications with HES5 and JAG2
 RBPJ antibody  >12 publications with HES5 and RBPJ
 NOTCH4 antibody  >8 publications with HES5 and NOTCH4
 MAML2 antibody  >4 publications with HES5 and MAML2
 JAK2 antibody  >2 publications with HES5 and JAK2
 MAML1 antibody  >2 publications with HES5 and MAML1

Pathways associated with anti-HES5 antibody

 Products by Pathway  Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 FBXW7 Mutants And NOTCH1 In Cancer Pathway antibodies  FBXW7 Mutants And NOTCH1 In Cancer Pathway Diagram
 NOTCH1 Intracellular Domain Regulates Transcription Pathway antibodies  NOTCH1 Intracellular Domain Regulates Transcription Pathway Diagram
 NOTCH2 Intracellular Domain Regulates Transcription Pathway antibodies  NOTCH2 Intracellular Domain Regulates Transcription Pathway Diagram
 Neural Crest Differentiation Pathway antibodies  Neural Crest Differentiation Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram

Diseases associated with anti-HES5 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >32 publications with HES5 and Nervous System Diseases
 Brain Diseases Antibodies  >29 publications with HES5 and Brain Diseases
 Mental Disorders Antibodies  >10 publications with HES5 and Mental Disorders
 Brain Neoplasms Antibodies  >8 publications with HES5 and Brain Neoplasms
 Inflammation Antibodies  >6 publications with HES5 and Inflammation
 Necrosis Antibodies  >5 publications with HES5 and Necrosis
 Hyperplasia Antibodies  >4 publications with HES5 and Hyperplasia
 Seizures Antibodies  >4 publications with HES5 and Seizures
 Neural Tube Defects Antibodies  >4 publications with HES5 and Neural Tube Defects
 Abnormalities, Multiple Antibodies  >3 publications with HES5 and Abnormalities, Multiple

Organs/Tissues associated with anti-HES5 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >120 publications with HES5 and Brain
 Embryonic Tissue Antibodies  >44 publications with HES5 and Embryonic Tissue
 Eye Antibodies  >23 publications with HES5 and Eye
 Lung Antibodies  >10 publications with HES5 and Lung
 Intestine Antibodies  >6 publications with HES5 and Intestine
 Heart Antibodies  >5 publications with HES5 and Heart
 Testis Antibodies  >3 publications with HES5 and Testis
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