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LIN54 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153711

LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell… More details

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Product Overview

Online Datasheet in PDF
Product Name
LIN54, Polyclonal Antibody
Full Product Name
LIN54 Antibody
Product Gene Name
anti-LIN54 antibody
Product Synonym Names
LIN-54; JC8.6; CXCDC1; MIP120; lin-54 homolog (C. elegans)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
LIN54 antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist.
Preparation and Storage
LIN54 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
LIN54 peptide (MBS153788) is used for blocking the activity of LIN54 antibody (MBS153711)
Molecular Weight
Predicted: 88 kDa

Observed: 82 kDa
OMIM
613367
Conjugate
Unconjugated
Immunogen
LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54.
Buffer
LIN54 antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-LIN54 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-LIN54 antibody
LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 mug/ml.

Western Blot (WB) of anti-LIN54 antibody

Western blot analysis of LIN54 in HeLa cell lysate with LIN54 antibody at 1 μg/ml.
Rabbit Anti-LIN54 Polyclonal Antibody – Cat# MBS153711

Immunocytochemistry (IHC) of anti-LIN54 antibody

Immunocytochemistry of LIN54 in HeLa cells with LIN54 at 2.5 μg/mL.
Rabbit Anti-LIN54 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS153711

Immunofluorescence (IF) of anti-LIN54 antibody

Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.
Rabbit Anti-LIN54 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153711

Immunocytochemistry (ICC) of anti-LIN54 antibody

Immunocytochemistry of LIN54 in HeLa cells with LIN54 antibody at 10 μg/ml.
Rabbit Anti-LIN54 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS153711

Immunofluorescence (IF) of anti-LIN54 antibody

Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.

Red: LIN54 Antibody (7933)
Blue: DAPI staining
Rabbit Anti-LIN54 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153711

Other Notes

Small volumes of anti-LIN54 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LIN54 purchase

MBS153711 is an antibody targeting LIN54. Also known as LIN54 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for LIN54. It may not necessarily be applicable to this product.

NCBI GI #
169234719
NCBI GeneID
132660
NCBI Accession #
NP_919258
UniProt Secondary Accession #
Q32M68; Q32M69; Q6N071; Q76B60
UniProt Related Accession #
Q6MZP7
GenBank Nucleotide #
NM_194282.3
NCBI Official Full Name
protein lin-54 homolog isoform a
NCBI Official Synonym Full Names
lin-54 DREAM MuvB core complex component
NCBI Official Symbol
LIN54
NCBI Official Synonym Symbols
JC8.6; CXCDC1; MIP120
UniProt Protein Name
Protein lin-54 homolog
UniProt Synonym Protein Names
CXC domain-containing protein 1
Protein Family
Protein
UniProt Gene Name
LIN54
UniProt Synonym Gene Names
CXCDC1; KIAA2037
UniProt Entry Name
LIN54_HUMAN
NCBI Protein Information
protein lin-54 homolog; CXC domain-containing protein 1

NCBI Summary for LIN54

LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes (Schmit et al., 2009 [PubMed 19725879]).[supplied by OMIM, Dec 2010]

UniProt Comments for LIN54

DKFZp686L1814: Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner. Belongs to the lin-54 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 4q21.22

Cellular Component: nucleoplasm

Molecular Function: protein binding; DNA binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of cell cycle; mitotic cell cycle; G2/M transition of mitotic cell cycle
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Research Articles on LIN54

1. LIN54 harboring a mutation in CHC domain is localized to the cytoplasm and inhibits cell cycle progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LIN54 antibody

 Reference Product  PubMed Publications
 LIN9 antibody  >4 publications with LIN54 and LIN9
 E2F4 antibody  >3 publications with LIN54 and E2F4
 LIN37 antibody  >3 publications with LIN54 and LIN37
 RBBP4 antibody  >3 publications with LIN54 and RBBP4
 LIN52 antibody  >3 publications with LIN54 and LIN52
 RBL2 antibody  >1 publications with LIN54 and RBL2

Pathways associated with anti-LIN54 antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 G0 And Early G1 Pathway antibodies  G0 And Early G1 Pathway Diagram
 G2/M Transition Pathway antibodies  G2/M Transition Pathway Diagram
 Mitotic G1-G1/S Phases Pathway antibodies  Mitotic G1-G1/S Phases Pathway Diagram
 Mitotic G2-G2/M Phases Pathway antibodies  Mitotic G2-G2/M Phases Pathway Diagram
 Polo-like Kinase Mediated Events Pathway antibodies  Polo-like Kinase Mediated Events Pathway Diagram

Organs/Tissues associated with anti-LIN54 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with LIN54 and Brain
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