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AKAP4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153720

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell (1). AKAP4,… More details

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Product Overview

Online Datasheet in PDF
Product Name
AKAP4, Polyclonal Antibody
Full Product Name
AKAP4 Antibody
Product Gene Name
Product Synonym Names
A-kinase anchor protein 4; AKAP-4; AKAP82; hAKAP82; CT99; FSC1; HI; PRKA4; A kinase (PRKA) anchor protein 4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
AKAP4 antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.
Preparation and Storage
AKAP4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Molecular Weight
Predicted: 94 kDa

Observed: 100 kDa
OMIM
300185
3D Structure
ModBase 3D Structure for Q5JQC9
Conjugate
Unconjugated
Immunogen
AKAP4 antibody was raised against a 17 amino acid peptide near the amino terminus of human AKAP4.
Buffer
AKAP4 antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-AKAP4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-AKAP4 antibody
AKAP4 antibody can be used for detection of AKAP4 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-AKAP4 antibody

Western blot analysis of AKAP4 in human testis tissue lysate with AKAP4 antibody at (A) 1 and (B) 2 μg/ml.
Rabbit Anti-AKAP4 Polyclonal Antibody – Cat# MBS153720

Immunohistochemistry (IHC) of anti-AKAP4 antibody

Immunohistochemistry of AKAP4 in human testis tissue with AKAP4 antibody at 5 μg/ml.
Rabbit Anti-AKAP4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153720

Immunofluorescence (IF) of anti-AKAP4 antibody

Immunofluorescence of AKAP4 in human testis tissue with AKAP4 antibody at 20 μg/ml.
Rabbit Anti-AKAP4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS153720

Other Notes

Small volumes of anti-AKAP4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKAP4 purchase

MBS153720 is an antibody targeting AKAP4. Also known as AKAP4 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AKAP4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
A-kinase anchor protein 4 isoform 1
NCBI Official Synonym Full Names
A kinase (PRKA) anchor protein 4
NCBI Official Symbol
AKAP4
NCBI Official Synonym Symbols
HI; p82; CT99; FSC1; PRKA4; AKAP-4; AKAP82; AKAP 82; hAKAP82
UniProt Protein Name
A-kinase anchor protein 4
UniProt Synonym Protein Names
A-kinase anchor protein 82 kDa; AKAP 82; hAKAP82; Major sperm fibrous sheath protein; HI; Protein kinase A-anchoring protein 4; PRKA4
UniProt Gene Name
AKAP4
UniProt Synonym Gene Names
AKAP-4; AKAP 82; hAKAP82; HI; PRKA4
UniProt Entry Name
AKAP4_HUMAN
NCBI Protein Information
A-kinase anchor protein 4; AKAP4; testis-specific gene HI; cancer/testis antigen 99; A-kinase anchor protein 82 kDa; major sperm fibrous sheath protein; protein kinase A anchoring protein 4; protein kinase A-anchoring protein 4

NCBI Summary for AKAP4

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is localized to the sperm flagellum and may be involved in the regulation of sperm motility. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for AKAP4

AKAP4: Major structural component of sperm fibrous sheath. Plays a role in sperm motility. Belongs to the AKAP110 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoskeleton; perinuclear region of cytoplasm; cytoplasm; nucleus; Z disc; cAMP-dependent protein kinase complex

Molecular Function: protein kinase A binding

Biological Process: protein localization; sperm motility; cell projection organization and biogenesis; single fertilization; transmembrane receptor protein serine/threonine kinase signaling pathway; cell motility; signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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