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SMURF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS153727

SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1… More details

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Product Overview

Online Datasheet in PDF
Product Name
SMURF1, Polyclonal Antibody
Full Product Name
SMURF1 Antibody
Product Gene Name
anti-SMURF1 antibody
Product Synonym Names
SMAD specific E3 ubiquitin protein ligase 1; SMAD ubiquitination regulatory factor 1; E3 ubiquitin-protein ligase SMURF1; SMAD specific E3 ubiquitin protein ligase 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
SMURF1 antibody is affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.
Preparation and Storage
SMURF1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
SMURF1 peptide (MBS154064) is used for blocking the activity of SMURF1 antibody (MBS153727)
Molecular Weight
Predicted: 80; 83 kDa

Observed: 78 kDa
OMIM
605568
Conjugate
Unconjugated
Immunogen
SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1.
Buffer
SMURF1 antibody is supplied in PBS containing 0.02% sodium azide.
Applications Tested/Suitable for anti-SMURF1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SMURF1 antibody
SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 mug/mL.

Western Blot (WB) of anti-SMURF1 antibody

Western blot analysis of SMURF1 in 293 cell lysate with SMURF1 antibody at 1 μg/ml.
Rabbit Anti-SMURF1 Polyclonal Antibody – Cat# MBS153727

Immunohistochemistry (IHC) of anti-SMURF1 antibody

Immunohistochemistry of SMURF1 in rat kidney tissue with SMURF1 antibody at 5 μg/ml.
Rabbit Anti-SMURF1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS153727

Other Notes

Small volumes of anti-SMURF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMURF1 purchase

MBS153727 is an antibody targeting SMURF1. Also known as SMURF1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SMURF1. It may not necessarily be applicable to this product.

NCBI GI #
31317292
NCBI GeneID
57154
NCBI Accession #
NP_065162
UniProt Secondary Accession #
O75853; Q547Q3; Q9UJT8; A4D279; B7ZMB6; B9EGV3
UniProt Related Accession #
Q9HCE7
GenBank Nucleotide #
NM_020429.2
NCBI Official Full Name
E3 ubiquitin-protein ligase SMURF1 isoform 1
NCBI Official Synonym Full Names
SMAD specific E3 ubiquitin protein ligase 1
NCBI Official Symbol
SMURF1
UniProt Protein Name
E3 ubiquitin-protein ligase SMURF1
UniProt Synonym Protein Names
SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
SMURF1
UniProt Synonym Gene Names
KIAA1625; hSMURF1
UniProt Entry Name
SMUF1_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase SMURF1; hSMURF1; E3 ubiquitin ligase SMURF1; Smad-specific E3 ubiquitin ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1

NCBI Summary for SMURF1

This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]
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UniProt Comments for SMURF1

SMURF1: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; cell soma; axon; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; ubiquitin-protein ligase activity; activin binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; ubiquitin-dependent SMAD protein catabolic process; negative regulation of BMP signaling pathway; BMP signaling pathway; receptor catabolic process; transforming growth factor beta receptor signaling pathway; ectoderm development; negative regulation of ossification; protein export from nucleus; negative regulation of transforming growth factor beta receptor signaling pathway; cell differentiation
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Research Articles on SMURF1

1. association of cortactin with Pfn-1 is regulated by c-Abl-mediated cortactin phosphorylation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SMURF1 antibody

 Reference Product  PubMed Publications
 SMURF2 antibody  >48 publications with SMURF1 and SMURF2
 RHOA antibody  >37 publications with SMURF1 and RHOA
 SMAD1 antibody  >37 publications with SMURF1 and SMAD1
 SMAD7 antibody  >32 publications with SMURF1 and SMAD7
 SMAD2 antibody  >22 publications with SMURF1 and SMAD2
 SMAD5 antibody  >17 publications with SMURF1 and SMAD5
 SMAD4 antibody  >16 publications with SMURF1 and SMAD4
 SMAD6 antibody  >15 publications with SMURF1 and SMAD6

Pathways associated with anti-SMURF1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Asymmetric Localization Of PCP Proteins Pathway antibodies  Asymmetric Localization Of PCP Proteins Pathway Diagram
 BMP Receptor Signaling Pathway antibodies  BMP Receptor Signaling Pathway Diagram
 BMP Signalling And Regulation Pathway antibodies  BMP Signalling And Regulation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downregulation Of TGF-beta Receptor Signaling Pathway antibodies  Downregulation Of TGF-beta Receptor Signaling Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram
 Endocytosis Pathway antibodies  Endocytosis Pathway Diagram

Diseases associated with anti-SMURF1 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >10 publications with SMURF1 and Necrosis
 Fibrosis Antibodies  >8 publications with SMURF1 and Fibrosis
 Inflammation Antibodies  >8 publications with SMURF1 and Inflammation
 Breast Neoplasms Antibodies  >7 publications with SMURF1 and Breast Neoplasms
 Liver Diseases Antibodies  >5 publications with SMURF1 and Liver Diseases
 Liver Neoplasms Antibodies  >5 publications with SMURF1 and Liver Neoplasms
 Kidney Diseases Antibodies  >4 publications with SMURF1 and Kidney Diseases
 Heart Diseases Antibodies  >3 publications with SMURF1 and Heart Diseases
 Nervous System Diseases Antibodies  >3 publications with SMURF1 and Nervous System Diseases
 Lung Neoplasms Antibodies  >3 publications with SMURF1 and Lung Neoplasms

Organs/Tissues associated with anti-SMURF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >77 publications with SMURF1 and Bone
 Muscle Antibodies  >13 publications with SMURF1 and Muscle
 Connective Tissue Antibodies  >11 publications with SMURF1 and Connective Tissue
 Embryonic Tissue Antibodies  >11 publications with SMURF1 and Embryonic Tissue
 Kidney Antibodies  >10 publications with SMURF1 and Kidney
 Heart Antibodies  >9 publications with SMURF1 and Heart
 Liver Antibodies  >8 publications with SMURF1 and Liver
 Bone Marrow Antibodies  >7 publications with SMURF1 and Bone Marrow
 Brain Antibodies  >6 publications with SMURF1 and Brain
 Lung Antibodies  >5 publications with SMURF1 and Lung
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