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GOLGIN97 (GOLGA1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154359

GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren's syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1,2). GOLGIN97… More details

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Product Overview

Online Datasheet in PDF
Product Name
GOLGIN97 (GOLGA1), Polyclonal Antibody
Full Product Name
GOLGIN97 Antibody
Product Gene Name
Product Synonym Names
Golgin subfamily A member 1, GOLGA1, GOLGIN A1, GOLGIN-97
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
GOLGIN97 peptide (MBS154427) is used for blocking the activity of GOLGIN97 antibody (MBS154359)
Molecular Weight
Predicted: 84 kDa
Observed: 120 kDa
OMIM
602502
3D Structure
ModBase 3D Structure for Q92805
Immunogen
GOLGIN97 antibody was raised against a 17 amino acid peptide near the amino terminus of human GOLGIN97.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
Human Stomach Tissue Lysate
Conjugate
Unconjugated
MW Explanation
Heavily post-translationally modifed, multiple independent GOLGIN97 antibodies show bands at ~120kDa

Product Categories/Family for anti-GOLGA1 antibody

Applications Tested/Suitable for anti-GOLGA1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-GOLGA1 antibody
GOLGIN97 antibody can be used for detection of GOLGIN97 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-GOLGA1 antibody

Western blot analysis of GOLGIN97 in human stomach tissue lysate with GOLGIN97 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking petpide.
Rabbit Anti-GOLGA1 Polyclonal Antibody – Cat# MBS154359

Immunohistochemistry (IHC) of anti-GOLGA1 antibody

Immunohistochemistry of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 5 μg/mL.
Rabbit Anti-GOLGA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154359

Immunofluorescence (IF) of anti-GOLGA1 antibody

Immunofluorescence of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 20 μg/mL.
Rabbit Anti-GOLGA1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS154359

Product References and Citations for anti-GOLGA1 antibody

Griffith KJ, Chan EK, Lung CC, et al. Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjogren's syndrome. Arthritis Rheum. 1997; 40:1693-702. Borchers AT, Naguwa SM, Keen CL, et al. Immunopathogenesis of Sjogren's syndrome. Clin. Rev. Allergy Immunol. 2003; 25:89-104. Lu L, Tai G, and Hong W. Autoantigen Golgin-97, and effector of ARL1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Mol. Biol. Cell 2004; 15:4426-43. Jing J, Junutula JR, Wu C, et al. FIP1/RCP binding to Golgin-97 regulates retrograde transport from recycling endosomes to the trans-Golgi network. Mol. Biol. Cell 2010; 21:3041-53.

Other Notes

Small volumes of anti-GOLGA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOLGA1 purchase

MBS154359 is an antibody targeting GOLGA1. Also known as GOLGIN97 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for GOLGA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
golgin subfamily A member 1
NCBI Official Synonym Full Names
golgin A1
NCBI Official Symbol
GOLGA1
NCBI Official Synonym Symbols
golgin-97
UniProt Protein Name
Golgin subfamily A member 1
UniProt Synonym Protein Names
Golgin-97
Protein Family
UniProt Gene Name
GOLGA1
UniProt Entry Name
GOGA1_HUMAN
NCBI Protein Information
golgin subfamily A member 1

NCBI Summary for GOLGA1

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein is associated with Sjogren's syndrome. [provided by RefSeq, Feb 2010]
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UniProt Comments for GOLGA1

GOLGA1: Probably involved in maintaining Golgi structure.

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; perinuclear region of cytoplasm; trans-Golgi network

Molecular Function: protein binding

Biological Process: protein targeting to Golgi
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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