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IZUMO1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154365

IZUMO1, a sperm-specific protein, is essential for sperm-egg plasma membrane binding and fusion. It is involved in the fertilization process and in the fusion of the sperm with the egg (1,2). IZUMO1 is expressed as at least four isoforms produced by… More details

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Product Overview

Online Datasheet in PDF
Product Name
IZUMO1, Polyclonal Antibody
Full Product Name
IZUMO1 Antibody
Product Gene Name
Product Synonym Names
Izumo sperm-egg fusion 1, IZUMO, Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
IZUMO1 antibody is human specific. At least four isoforms of IZUMO1 are known to exist; this antibody will detect the two longest isoforms.
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
IZUMO1 peptide (MBS154397) is used for blocking the activity of IZUMO1 antibody (MBS154365)
Molecular Weight
Predicted: 35, 39, 42 kDa
Observed: 52 kDa
OMIM
609278
3D Structure
ModBase 3D Structure for Q8IYV9
Immunogen
IZUMO1 antibody was raised against an 18 amino acid peptide near the center of human IZUMO1
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
Human Testis Tissue Lysate
Conjugate
Unconjugated
MW Explanation
Transmembrane protein, also multiple other IZUMO1 antibodies also show a ~52kD band

Product Categories/Family for anti-IZUMO1 antibody

Applications Tested/Suitable for anti-IZUMO1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-IZUMO1 antibody
IZUMO1 antibody can be used for detection of IZUMO1 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.

Western Blot (WB) of anti-IZUMO1 antibody

Western blot analysis of IZUMO1 in human testis tissue lysate with IZUMO1 antibody at 1 μg/ml.
Rabbit Anti-IZUMO1 Polyclonal Antibody – Cat# MBS154365

Immunohistochemistry (IHC) of anti-IZUMO1 antibody

Immunohistochemistry of IZUMO1 in human testis tissue with IZUMO1 antibody at 2.5 μg/mL.
Rabbit Anti-IZUMO1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154365

Product References and Citations for anti-IZUMO1 antibody

Inoue N, Hamada D, Kamikubo H, et al. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development 2013; 140:3221-9. Inoue N, Ikawa M, and Okabe M. The mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion. Asian J. Androl. 2011; 13:81-7. Xing WJ, Han BD, Wu Q, et al. Molecular cloning and characterization of Izumo1 gene from sheep and cashmere goat reveal alternative splicing. Mol. Biol. Rep. 2011; 38:1995-2006. Inoue N, Ikawa M, Isotani A, et al. The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature 2005; 434:234-8.

Other Notes

Small volumes of anti-IZUMO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IZUMO1 purchase

MBS154365 is an antibody targeting IZUMO1. Also known as IZUMO1 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for IZUMO1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
izumo sperm-egg fusion protein 1 isoform 1
NCBI Official Synonym Full Names
izumo sperm-egg fusion 1
NCBI Official Symbol
IZUMO1
NCBI Official Synonym Symbols
OBF; IZUMO
UniProt Protein Name
Izumo sperm-egg fusion protein 1
UniProt Synonym Protein Names
Oocyte binding/fusion factor; OBF; Sperm-specific protein izumo
UniProt Gene Name
IZUMO1
UniProt Synonym Gene Names
OBF
UniProt Entry Name
IZUM1_HUMAN
NCBI Protein Information
izumo sperm-egg fusion protein 1

NCBI Summary for IZUMO1

The sperm-specific protein Izumo, named for a Japanese shrine dedicated to marriage, is essential for sperm-egg plasma membrane binding and fusion (Inoue et al., 2005 [PubMed 15759005]).[supplied by OMIM, Mar 2008]

UniProt Comments for IZUMO1

IZUMO1: Involved in the fertilization process. May be involved in the fusion of the sperm with the egg. Belongs to the Izumo family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: acrosomal membrane; integral to membrane; plasma membrane

Molecular Function: protein homodimerization activity; receptor binding

Biological Process: cell adhesion; fusion of sperm to egg plasma membrane; single fertilization; sperm-egg recognition
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IZUMO1 antibody

 Reference Product  PubMed Publications
 CD9 antibody  >16 publications with IZUMO1 and CD9

Organs/Tissues associated with anti-IZUMO1 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >13 publications with IZUMO1 and Testis
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