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TRPC3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154368

The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to… More details

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Product Overview

Online Datasheet in PDF
Product Name
TRPC3, Polyclonal Antibody
Full Product Name
TRPC3 Antibody
Product Gene Name
Product Synonym Names
Transient receptor potential cation channel, subfamily C, member 3; TRP3; TRP3; Short transient receptor potential channel 3; Transient receptor protein 3; TrpC3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
TRPC3 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect the three largest isoforms. TRPC3 antibody is predicted to not cross-react with TRPC6.
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
TRPC3 peptide (MBS154410) is used for blocking the activity of TRPC3 antibody (MBS154368)
Molecular Weight
Predicted: 90, 93, 101 kDa
Observed: 93 kDa
OMIM
602345
3D Structure
ModBase 3D Structure for Q13507
Immunogen
TRPC3 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TRPC3.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
Human Cerebellum Tissue Lysate
Conjugate
Unconjugated
Applications Tested/Suitable for anti-TRPC3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TRPC3 antibody
TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 mug/mL.Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.

Western Blot (WB) of anti-TRPC3 antibody

Western blot analysis of TRPC3 in human cerebellum tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/ml.
Rabbit Anti-TRPC3 Polyclonal Antibody – Cat# MBS154368

Immunohistochemistry (IHC) of anti-TRPC3 antibody

Immunohistochemistry of TRPC3 in human brain tissue with TRPC3 antibody at 2.5 μg/mL.
Rabbit Anti-TRPC3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154368

Product References and Citations for anti-TRPC3 antibody

Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31. Trebak M, Vazquez G, Bird GSJ, et al. The TRPC3/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61. Zitt C, Obukhov AG, Strubing C, et al. Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion. J. Cell. Biol. 1997; 1333-41. Dietrich A, Kalwa H, Rost BR, et al. The diacylglycerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch. 2005; 451:72-80.

Other Notes

Small volumes of anti-TRPC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRPC3 purchase

MBS154368 is an antibody targeting TRPC3. Also known as TRPC3 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TRPC3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
short transient receptor potential channel 3 isoform a
NCBI Official Synonym Full Names
transient receptor potential cation channel subfamily C member 3
NCBI Official Symbol
TRPC3
NCBI Official Synonym Symbols
TRP3; SCA41
UniProt Protein Name
Short transient receptor potential channel 3
UniProt Synonym Protein Names
Transient receptor protein 3; TRP-3; hTrp-3; hTrp3
UniProt Gene Name
TRPC3
UniProt Synonym Gene Names
TRP3; TrpC3; TRP-3; hTrp-3; hTrp3
UniProt Entry Name
TRPC3_HUMAN
NCBI Protein Information
short transient receptor potential channel 3

NCBI Summary for TRPC3

The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for TRPC3

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol 1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-TRPC3 antibody

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