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RFX1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154374

RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1,2).… More details

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Product Overview

Online Datasheet in PDF
Product Name
RFX1, Polyclonal Antibody
Full Product Name
RFX1 Antibody
Product Gene Name
anti-RFX1 antibody
Product Synonym Names
Regulatory factor X, 1 (influences HLA class II expression); RFX; MHC class II regulatory factor RFX; MHC class II regulatory factor RFX1; enhancer factor C; EFC; trans-acting regulatory factor 1; transcription factor RFX1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Purity/Purification
Affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
RFX1 peptide (MBS154395) is used for blocking the activity of RFX1 antibody (MBS154374)
Molecular Weight
Predicted: 108 kDa
Observed: 125 kDa
OMIM
600006
3D Structure
ModBase 3D Structure for P22670
Immunogen
RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
HeLa Cell Lysate
Conjugate
Unconjugated
MW Explanation
Multiple independent RFX1 antibodies also show a band at ~130 kDa

Product Categories/Family for anti-RFX1 antibody

Immunology; Autophagy
Applications Tested/Suitable for anti-RFX1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-RFX1 antibody
RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 mug/ml.

Western Blot (WB) of anti-RFX1 antibody

Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 μg/ml.
Rabbit Anti-RFX1 Polyclonal Antibody – Cat# MBS154374

Immunocytochemistry (IHC) of anti-RFX1 antibody

Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 μg/mL.
Rabbit Anti-RFX1 Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS154374

Immunofluorescence (IF) of anti-RFX1 antibody

Immunofluorescence of RFX1 in Hela cells with RFX1 antibody at 20 μg/mL.

Green: RFX1 Antibody (8199)
Blue: DAPI staining
Rabbit Anti-RFX1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS154374

Immunocytochemistry (ICC) of anti-RFX1 antibody

Immunocytochemistry of RFX1 in Hela cells with RFX1 antibody at 5 μg/mL.
Rabbit Anti-RFX1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS154374

Product References and Citations for anti-RFX1 antibody

Reith W, Ucla C, Barras E, et al. RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins. Mol. Cell Biol. 1994; 14:1230-44. Katan-Khaykovich Y and Shaul Y. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes. J. Biol. Chem. 1998; 273:24504-12. Hsu YC, Liao WC, Kao CY, et al. Regulation of FGF1 gene promoter through transcription factor RFX1. J. Biol. Chem. 2010; 285:13885-95. Su JC, Tseng PH, Hsu CY, et al. RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells. Oncotarget 2014; 5:4909-19.

Other Notes

Small volumes of anti-RFX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RFX1 purchase

MBS154374 is an antibody targeting RFX1. Also known as RFX1 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RFX1. It may not necessarily be applicable to this product.

NCBI GI #
238859557
NCBI GeneID
5989
NCBI Accession #
NP_002909
UniProt Primary Accession #
P22670
UniProt Related Accession #
P22670
GenBank Nucleotide #
NM_002918.4
NCBI Official Full Name
MHC class II regulatory factor RFX1
NCBI Official Synonym Full Names
regulatory factor X1
NCBI Official Symbol
RFX1
NCBI Official Synonym Symbols
EFC; RFX
UniProt Protein Name
MHC class II regulatory factor RFX1
UniProt Synonym Protein Names
Enhancer factor C; EF-C; Regulatory factor X 1; RFX; Transcription factor RFX1
Protein Family
MHC class II regulatory factor
UniProt Gene Name
RFX1
UniProt Synonym Gene Names
EF-C; RFX
UniProt Entry Name
RFX1_HUMAN
NCBI Protein Information
MHC class II regulatory factor RFX1

NCBI Summary for RFX1

This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. [provided by RefSeq, Jul 2008]
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UniProt Comments for RFX1

RFX1: Regulatory factor essential for MHC class II genes expression. Binds to the X boxes of MHC class II genes. Also binds to an inverted repeat (ENH1) required for hepatitis B virus genes expression and to the most upstream element (alpha) of the RPL30 promoter. Belongs to the RFX family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: protein binding; RNA polymerase II transcription factor activity, enhancer binding

Biological Process: immune response; regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
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Research Articles on RFX1

1. Data indicate that regulatory factor X transcription factor RFX-1 regulates SC-2001-mediated SHP-1 Phosphatase transcription in hepatocellular carcinoma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-RFX1 antibody

 Disease Name  Pubmed Publications
 Hepatitis Antibodies  >10 publications with RFX1 and Hepatitis
 Liver Neoplasms Antibodies  >4 publications with RFX1 and Liver Neoplasms
 Brain Diseases Antibodies  >4 publications with RFX1 and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >3 publications with RFX1 and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >3 publications with RFX1 and Liver Diseases
 Cell Transformation, Neoplastic Antibodies  >2 publications with RFX1 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >1 publications with RFX1 and Breast Neoplasms
 Neurobehavioral Manifestations Antibodies  >1 publications with RFX1 and Neurobehavioral Manifestations
 Embryo Loss Antibodies  >1 publications with RFX1 and Embryo Loss
 Fetal Death Antibodies  >1 publications with RFX1 and Fetal Death

Organs/Tissues associated with anti-RFX1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >11 publications with RFX1 and Brain
 Testis Antibodies  >9 publications with RFX1 and Testis
 Liver Antibodies  >8 publications with RFX1 and Liver
 Lung Antibodies  >3 publications with RFX1 and Lung
 Skin Antibodies  >2 publications with RFX1 and Skin
 Eye Antibodies  >2 publications with RFX1 and Eye
 Embryonic Tissue Antibodies  >2 publications with RFX1 and Embryonic Tissue
 Bone Antibodies  >1 publications with RFX1 and Bone
 Bone Marrow Antibodies  >1 publications with RFX1 and Bone Marrow
 Kidney Antibodies  >1 publications with RFX1 and Kidney
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