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RAET1E Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154386

The Retinoic acid early transcript 1E (RAET1E) belongs to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3 (1). Like the related protein RAET1G, RAET1E differs… More details

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Product Overview

Online Datasheet in PDF
Product Name
RAET1E, Polyclonal Antibody
Full Product Name
RAET1E Antibody
Product Gene Name
Product Synonym Names
Retinoic acid early transcript 1E, NKG2D ligand 4, N2DL-4, NKG2DL42, RL-4, ULBP4, LETAL
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid
Purity/Purification
Affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
RAET1E antibody is human and mouse reactive. At least four isoforms of RAET1E are known to exist; this antibody will detect all four.
Preparation and Storage
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
RAET1E peptide (MBS154428) is used for blocking the activity of RAET1E antibody (MBS154386)
Molecular Weight
Predicted: 29 kDa
Observed: 29 kDa
OMIM
609243
3D Structure
ModBase 3D Structure for P84103
Immunogen
RAET1E antibody was raised against an 18 amino acid peptide near the center of human RAET1E.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
EL4 Cell Lysate
Conjugate
Unconjugated

Product Categories/Family for anti-RAET1E antibody

Applications Tested/Suitable for anti-RAET1E antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-RAET1E antibody
RAET1E antibody can be used for detection of RAET1E by Western blot at 1 - 2 mug/ml.

Western Blot (WB) of anti-RAET1E antibody

Western blot analysis of RAET1E in EL4 cell lysate with RAET1E antibody at 1 μg/ml.
Rabbit Anti-RAET1E Polyclonal Antibody – Cat# MBS154386

Immunocytochemistry (IHC) of anti-RAET1E antibody

Immunocytochemistry of RAET1E in K562 cells with RAET1E at 2.5 μg/mL.
Rabbit Anti-RAET1E Polyclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS154386

Product References and Citations for anti-RAET1E antibody

Radosavlijevic M, Cuillerier B, Wilson MJ, et al. A cluster of ten novel MHC class I related genes on human chromosome 6q24.2-q25.3. Genomics 2002; 79:114-23. Bacon L, Eagle RA, Meyer M, et al. Two human ULBP/RAET1 molecules with transmembrane regions are ligands for NKG2D. J. Immunol. 2004; 173:1078-84. Letal, a tumor-associated NKG2D immunoreceptor ligand, induces activation and expansion of effector immune cells. Cancer Biol. Ther. 2003; 2:446-51.

Other Notes

Small volumes of anti-RAET1E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RAET1E purchase

MBS154386 is an antibody targeting RAET1E. Also known as RAET1E Antibody. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RAET1E. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
NKG2D ligand 4 isoform 1
NCBI Official Synonym Full Names
retinoic acid early transcript 1E
NCBI Official Symbol
RAET1E
NCBI Official Synonym Symbols
RL-4; LETAL; ULBP4; N2DL-4; NKG2DL4; RAET1E2; bA350J20.7
UniProt Protein Name
NKG2D ligand 4
UniProt Synonym Protein Names
Lymphocyte effector toxicity activation ligand; RAE-1-like transcript 4; RL-4; Retinoic acid early transcript 1E
Protein Family
UniProt Gene Name
RAET1E
UniProt Synonym Gene Names
LETAL; N2DL4; ULBP4; N2DL-4; NKG2DL4; RL-4
UniProt Entry Name
N2DL4_HUMAN
NCBI Protein Information
NKG2D ligand 4

NCBI Summary for RAET1E

This gene belong to the RAET1 family, which consists of major histocompatibility complex (MHC) class I-related genes located in a cluster on chromosome 6q24.2-q25.3. This and RAET1G protein differ from other RAET1 proteins in that they have type I membrane-spanning sequences at their C termini rather than glycosylphosphatidylinositol anchor sequences. This protein functions as a ligand for NKG2D receptor, which is expressed on the surface of several types of immune cells, and is involved in innate and adaptive immune responses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]
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UniProt Comments for RAET1E

RAET1E: Ligand for the NKG2D receptor. Delivers activating signals to NK cells and promotes tumor immune surveillance by inducing the expansion of anti-tumor cytotoxic lymphocytes. Belongs to the MHC class I family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: extracellular region; integral to membrane; plasma membrane

Molecular Function: antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell mediated cytotoxicity; regulation of immune response; T cell mediated cytotoxicity
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Research Articles on RAET1E

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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