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PMEPA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154391

The prostate transmembrane protein, androgen induced 1 (PMEPA1) protein is a transmembrane protein that contains a Smad interacting motif (SIM) (1,2). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein… More details

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Product Overview

Online Datasheet in PDF
Product Name
PMEPA1, Polyclonal Antibody
Full Product Name
PMEPA1 Antibody
Product Gene Name
Product Synonym Names
Prostate transmembrane protein androgen induced 1, STAG1, TMEPAI
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Specificity
PMEPA1 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all of the isoforms.
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Antibody/Peptide Pairs
PMEPA1 peptide (MBS154429) is used for blocking the activity of PMEPA1 antibody (MBS154391)
Molecular Weight
Predicted: 26, 28, 32 kDa
Observed: 27 kDa
OMIM
606564
3D Structure
ModBase 3D Structure for Q969W9
Immunogen
PMEPA1 antibody was raised against a 16 amino acid peptide near the center of human PMEPA1.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
A549 Cell Lysate
Conjugate
Unconjugated

Product Categories/Family for anti-PMEPA1 antibody

Applications Tested/Suitable for anti-PMEPA1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PMEPA1 antibody
PMEPA1 antibody can be used for detection of PMEPA1 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.

Western Blot (WB) of anti-PMEPA1 antibody

Western blot analysis of PMEPA1 inA549 cell lysate with PMEPA1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-PMEPA1 Polyclonal Antibody – Cat# MBS154391

Immunohistochemistry (IHC) of anti-PMEPA1 antibody

Immunohistochemistry of PMEPA1 in human prostate tissue with PMEPA1 antibody at 2.5 μg/ml.
Rabbit Anti-PMEPA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154391

Product References and Citations for anti-PMEPA1 antibody

Xu LL, Shanmugam N, Segawa T, et al. A novel androgen-regulated gene, PMEPA1, located on chromosome 20q!3 exhibits high level expression in prostate. Genomics 2000; 66:257-63. Rae FK, Hooper JD, Nicol DL, et al. Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors. Mol. Carcinog. 2001; 32:44-53. Watanabe Y, Itoh S, Goto T, et al. TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad proteins from active participation in TGF-beta signaling. Mol. Cell 2010; 37:123-34. Vo Nguyen TT, Watanabe Y, Shiba A, et al. TMEPAI/PMEPA1 enhances tumorigenic activities in lung cancer cells. Cancer Sci. 2014; 105:334-41.

Other Notes

Small volumes of anti-PMEPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PMEPA1 purchase

MBS154391 is an antibody targeting PMEPA1. Also known as PMEPA1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PMEPA1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein TMEPAI isoform a
NCBI Official Synonym Full Names
prostate transmembrane protein, androgen induced 1
NCBI Official Symbol
PMEPA1
NCBI Official Synonym Symbols
STAG1; TMEPAI
UniProt Protein Name
Protein TMEPAI
UniProt Synonym Protein Names
Prostate transmembrane protein androgen induced 1; Solid tumor-associated 1 protein; Transmembrane prostate androgen-induced protein
Protein Family
UniProt Gene Name
PMEPA1
UniProt Synonym Gene Names
STAG1; TMEPAI
UniProt Entry Name
PMEPA_HUMAN
NCBI Protein Information
protein TMEPAI

NCBI Summary for PMEPA1

This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
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UniProt Comments for PMEPA1

TMEPAI: Involved in down-regulation of the androgen receptor (AR). Enhances ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4. Belongs to the PMEPA1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.31-q13.33

Cellular Component: early endosome membrane; endosome membrane; Golgi membrane; integral to membrane; intracellular membrane-bound organelle; nucleoplasm; plasma membrane

Molecular Function: protein binding; WW domain binding

Biological Process: androgen receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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