Mouse Anti-VCL Monoclonal Antibody – Cat# MBS154524

Western blot analysis of Vinculin in 293, A431, A549, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and human kidney, human breast, mouse brain, mouse lung, mouse spleen, rat heart, rat lung, rat spleen, rat liver, rabbit spleen, rabbit brain and chicken spleen tissue lysate with Vinculin antibody at 1 μg/ml.

Vinculin (VCL) Antibody – Mouse Monoclonal

Name: VCL Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS154524
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154524

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal… More details

$530 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Vinculin (VCL), Monoclonal Antibody

Full Product Name

Vinculin Antibody [8B5]

Product Gene Name

anti-VCL antibody

Product Synonym Names

CMD1W; CMH15; Metavinculin; MVCL; vinculin

Clonality

Monoclonal

Isotype

IgG

Clone Number

8B5

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Rabbit, Chicken

Form/Format

Liquid

Purity/Purification

Protein A prification.

Concentration

1 mg/mL (lot specific)

Specificity

Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.

Preparation and Storage

Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Molecular Weight

Predicted: 117 kDa
Observed: 120 kDa

OMIM

M33308 mRNA

3D Structure

ModBase 3D Structure for P18206

Conjugate

Unconjugated

Immunogen

Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.

Buffer

Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.

Species

Human

Product Categories/Family for anti-VCL antibody

Homeostasis

Applications Tested/Suitable for anti-VCL antibody

ELISA (EIA), Western Blot (WB)

Application Notes for anti-VCL antibody

Western blot at 0.5 - 1 mug/ml.

Product References and Citations for anti-VCL antibody

1. Burridge K, Fath K, Kelly T, et al. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu. Rev. Cell Biol.1988; 4:487-525. 2. Gilmore AP, Jackson P, Waites GT, et al. Further characterization of the talin-binding site in the cytoskeletal protein vinculin. J. Cell Sci. 1992; 103:719-31. 3. Deakin NO, Ballestrem C, and Turner CE. Paxillin and Hic-5 interaction with vinculin is differentially regulated by Rac1 and RhoA. PLoS One 2012; 7:e37990. 4. Goldmann WH, Auernheimer V, Thievessen I, et al. Vinculin, cell mechanics and tumour cell invasion. Cell Biol. Int. 2013; Feb 1.

Other Notes

Small volumes of anti-VCL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VCL purchase

MBS154524 is an antibody targeting VCL. Also known as Vinculin Antibody [8B5]. Reactive in Human, Mouse, Rat, Rabbit, Chicken. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for VCL. It may not necessarily be applicable to this product.

NCBI GI #

4507877

NCBI GeneID

7414

NCBI Accession #

NP_003364

UniProt Primary Accession #

P18206

UniProt Secondary Accession #

Q16450; Q5SWX2; Q7Z3B8; Q8IXU7

UniProt Related Accession #

P18206

GenBank Nucleotide #

NM_003373.3

NCBI Official Full Name

vinculin isoform VCL

NCBI Official Synonym Full Names

vinculin

NCBI Official Symbol

VCL

NCBI Official Synonym Symbols

MV; MVCL; CMD1W; CMH15; HEL114

UniProt Protein Name

Vinculin

UniProt Synonym Protein Names

Metavinculin; MV

Protein Family

Vinculin

UniProt Gene Name

VCL

UniProt Synonym Gene Names

NCBI Protein Information

vinculin

NCBI Summary for VCL

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

UniProt Comments for VCL

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: adherens junction; cell-cell adherens junction; costamere; cytoskeleton; cytosol; extracellular region; focal adhesion; intercellular junction; protein complex

Molecular Function: actin binding; alpha-catenin binding; beta-catenin binding; cadherin binding; protein binding; ubiquitin protein ligase binding

Biological Process: apical junction assembly; cell adhesion; cell motility; cell-matrix adhesion; lamellipodium biogenesis; morphogenesis of an epithelium; muscle contraction; negative regulation of cell migration; neutrophil degranulation; platelet degranulation

Disease: Cardiomyopathy, Dilated, 1w

Research Articles on VCL

1. The VCL-encoding protein was involved in cardiomyopathy that associated with hypertension, therefore our results suggest the rs4746172 of VCL may be a novel target for clinical interventions to reduce CVD risk by regulating blood pressure in male Chinese

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VCL antibody

Reference Product	PubMed Publications
PTK2 antibody	>7 publications with VCL and PTK2
SRC antibody	>6 publications with VCL and SRC
PXN antibody	>3 publications with VCL and PXN
CTNNA1 antibody	>2 publications with VCL and CTNNA1
ACTN1 antibody	>2 publications with VCL and ACTN1
PRKCA antibody	>1 publications with VCL and PRKCA
GSN antibody	>1 publications with VCL and GSN

Pathways associated with anti-VCL antibody

Products by Pathway	Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with anti-VCL antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>33 publications with VCL and Cardiovascular Diseases
Neoplasms Antibodies	>26 publications with VCL and Neoplasms
Drug Toxicity Antibodies	>25 publications with VCL and Drug Toxicity
Nervous System Diseases Antibodies	>15 publications with VCL and Nervous System Diseases
Heart Diseases Antibodies	>15 publications with VCL and Heart Diseases
Brain Diseases Antibodies	>12 publications with VCL and Brain Diseases
Kidney Diseases Antibodies	>6 publications with VCL and Kidney Diseases
Neoplasm Metastasis Antibodies	>6 publications with VCL and Neoplasm Metastasis
Poisoning Antibodies	>5 publications with VCL and Poisoning
Lung Diseases Antibodies	>4 publications with VCL and Lung Diseases

Organs/Tissues associated with anti-VCL antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>47 publications with VCL and Blood
Muscle Antibodies	>44 publications with VCL and Muscle
Vascular Antibodies	>20 publications with VCL and Vascular
Heart Antibodies	>16 publications with VCL and Heart
Testis Antibodies	>16 publications with VCL and Testis
Brain Antibodies	>14 publications with VCL and Brain
Lung Antibodies	>12 publications with VCL and Lung
Liver Antibodies	>10 publications with VCL and Liver
Kidney Antibodies	>10 publications with VCL and Kidney
Bone Antibodies	>8 publications with VCL and Bone