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VISTA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154544

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
VISTA, Polyclonal Antibody
Full Product Name
VISTA Antibody
Product Gene Name
Product Synonym Gene Name
VSIR
Product Synonym Names
VISTA molecule; VSIR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; VISTA; C10orf54; chromosome 10 open reading frame 54; PD-1H; V-set immunoregulatory receptor; V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation; Chromosome 10 Open Reading Frame 54
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Supplied in PBS containing 0.02% sodium azide.
Purity/Purification
Affinity chromatography purified via peptide column.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
VISTA antibody can be stored at 4°C for three months and -20°C stable for up to one year. As with all antibodies care should be taken to avoid repeated freeeze/thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Antibody/Peptide Pairs
VISTA peptide (MBS154551) is used for blocking the activity of VISTA antibody (MBS154544)
OMIM
615608
3D Structure
ModBase 3D Structure for Q9H7M9
Immunogen
The immunogen is located within the last 60 amino acids of VISTA.
Conjugate
Unconjugated
Homology
Predicted Species reactivity based on immunogen sequence: Mouse: (93%).

VISTA antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human VISTA.

Product Categories/Family for anti-VISTA antibody

Applications Tested/Suitable for anti-VISTA antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-VISTA antibody
IHC at 2 ug/mL
IF start at 20 ug/mL
ICC start at 1 ug/mL.

Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples.
All other applications and species not yet tested.

Immunohistochemistry (IHC) of anti-VISTA antibody

Immunohistochemistry of VISTA in human tonsil tissue with B7-H5 antibody at 2 μg/ml.
Rabbit Anti-VISTA Polyclonal Antibody – Cat# MBS154544

Immunofluorescence (IF) of anti-VISTA antibody

Immunofluorescence of VISTA in human tonsil tissue with B7-H5 antibody at 20 μg/ml.
Rabbit Anti-VISTA Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS154544

Immunocytochemistry (ICC) of anti-VISTA antibody

Immunocytochemistry of VISTA in overexpressing HEK293 cells with VISTA antibody at 1 μg/ml.
Rabbit Anti-VISTA Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS154544

Product References and Citations for anti-VISTA antibody

Mayya V., et al. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46. Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.

Other Notes

Small volumes of anti-VISTA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VISTA purchase

MBS154544 is an antibody targeting VISTA. Also known as VISTA Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for VISTA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
NCBI Official Synonym Full Names
V-set immunoregulatory receptor
NCBI Official Symbol
VSIR
NCBI Official Synonym Symbols
B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
UniProt Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Synonym Protein Names
Platelet receptor Gi24
UniProt Gene Name
VSIR
UniProt Synonym Gene Names
Sisp-1
NCBI Protein Information
V-type immunoglobulin domain-containing suppressor of T-cell activation

UniProt Comments for VISTA

Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-VISTA antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1 publications with VISTA and Inflammation

Organs/Tissues associated with anti-VISTA antibody

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