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PDL2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154568

MBS154568 is an antibody targeting PDL2. Also known as PDL2 Antibody [7C1]. Reactive in Human. Host: Mouse. More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDL2, Monoclonal Antibody
Full Product Name
PDL2 Antibody [7C1]
Product Gene Name
anti-PDL2 antibody
Product Synonym Gene Name
PDCD1LG2
Product Synonym Names
B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2; B7DC; Programmed cell death 1 ligand 2; Butyrophilin B7-DC; PD-1 ligand 2; programmed cell death 1 ligand
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7C1
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid
Supplied in PBS containing 0.02% sodium azide and 50% glycerol
Purity/Purification
PD-L2 Antibody is supplied as protein A purified IgG2b
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 30 kDa
Observed: 38 kDa
OMIM
605723
3D Structure
ModBase 3D Structure for P9BQ51
Immunogen
PD-L2 antibody was raised against the extracellular domain of human PD-L2.
Conjugate
Unconjugated

Product Categories/Family for anti-PDL2 antibody

Immunology
Applications Tested/Suitable for anti-PDL2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-PDL2 antibody
Optimal dilutions for each application to be determined by the researcher

Western Blot (WB) of anti-PDL2 antibody

Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml
Mouse Anti-PDL2 Monoclonal Antibody – Cat# MBS154568

Immunocytochemistry (IHC) of anti-PDL2 antibody

Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.
Mouse Anti-PDL2 Monoclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS154568

Immunofluorescence (IF) of anti-PDL2 antibody

Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL.

Green: PDL2 Antibody [7C1]
Blue: DAPI staining
Mouse Anti-PDL2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154568

Immunofluorescence (IF) of anti-PDL2 antibody

Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL.

Green: PDL2 Antibody [7C1]
Blue: DAPI staining
Mouse Anti-PDL2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154568

Immunohistochemistry (IHC) of anti-PDL2 antibody

Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.
Mouse Anti-PDL2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154568

Immunohistochemistry (IHC) of anti-PDL2 antibody

Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.
Mouse Anti-PDL2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154568

Flow Cytometry (FC/FACS) of anti-PDL2 antibody

Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.
Mouse Anti-PDL2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS154568

Product References and Citations for anti-PDL2 antibody

Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78. Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.

Other Notes

Small volumes of anti-PDL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PDL2. It may not necessarily be applicable to this product.

NCBI GI #
190014605
NCBI GeneID
80380
NCBI Accession #
NP_079515
UniProt Primary Accession #
P9BQ51
UniProt Secondary Accession #
Q14CN8; Q5T7Z6; Q6JXL8; Q6JXL9
UniProt Related Accession #
Q9BQ51
GenBank Nucleotide #
NM_025239.3
NCBI Official Full Name
programmed cell death 1 ligand 2
NCBI Official Synonym Full Names
programmed cell death 1 ligand 2
NCBI Official Symbol
PDCD1LG2
NCBI Official Synonym Symbols
B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
UniProt Protein Name
Programmed cell death 1 ligand 2
UniProt Synonym Protein Names
Butyrophilin B7-DC; B7-DC; CD_antigen: CD273
UniProt Gene Name
PDCD1LG2
UniProt Synonym Gene Names
B7DC; CD273; PDCD1L2; PDL2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; B7-DC
NCBI Protein Information
programmed cell death 1 ligand 2

UniProt Comments for PDL2

Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production ().

Research Articles on PDL2

1. The biopsy tumor key protein measurements demonstrate substantial between-tumor variation in expression ratios of these proteins and suggest that programmed cell death 1 ligand 2 PD-L2 is present in some tumors at levels sufficient to contribute to programmed cell death-1 PD-1-dependent T-cell regulation and possibly to affect responses to PD-1- and programmed cell death 1 ligand 1 PD-L1-blocking drugs.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PDL2 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 PD-1 Signaling Pathway antibodies  PD-1 Signaling Pathway Diagram
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