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CD80 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154584

CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD80, Monoclonal Antibody
Full Product Name
CD80 Antibody [11C12]
Product Gene Name
Product Synonym Names
CD80 molecule; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
11C12
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid
CD80 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Purity/Purification
CD80 Antibody is supplied as protein A purified IgG1.
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 32 kDa
Observed: 50 kDa
OMIM
112203
3D Structure
ModBase 3D Structure for P33681
Immunogen
CD80 antibody was raised against the extracellular domain of human CD80.
Conjugate
Unconjugated
User Note
Optimal dilutions for each application to be determined by the researcher.

Product Categories/Family for anti-CD80 antibody

Applications Tested/Suitable for anti-CD80 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-CD80 antibody
WB at 1-2 ug/mL
IHC starting at 2-ug/mL
IF start at 5 ug/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Western Blot (WB) of anti-CD80 antibody

Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5 μg/ml
Mouse Anti-CD80 Monoclonal Antibody – Cat# MBS154584

Immunocytochemistry (IHC) of anti-CD80 antibody

Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Mouse Anti-CD80 Monoclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS154584

Immunofluorescence (IF) of anti-CD80 antibody

Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL.

Red: CD80 Antibody [11C12]
Blue: DAPI staining
Mouse Anti-CD80 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154584

Immunofluorescence (IF) of anti-CD80 antibody

Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL.

Red: CD80 Antibody [11C12]
Blue: DAPI staining
Mouse Anti-CD80 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154584

Product References and Citations for anti-CD80 antibody

Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400. Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

Other Notes

Small volumes of anti-CD80 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD80 purchase

MBS154584 is an antibody targeting CD80. Also known as CD80 Antibody [11C12]. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD80. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-lymphocyte activation antigen CD80
NCBI Official Synonym Full Names
CD80 molecule
NCBI Official Symbol
CD80
NCBI Official Synonym Symbols
B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
UniProt Protein Name
T-lymphocyte activation antigen CD80
UniProt Synonym Protein Names
Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD_antigen: CD80
UniProt Gene Name
CD80
UniProt Synonym Gene Names
CD28LG; CD28LG1; LAB7; B7
NCBI Protein Information
T-lymphocyte activation antigen CD80

NCBI Summary for CD80

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
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UniProt Comments for CD80

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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