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TIGIT Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154593

The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind… More details

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Product Overview

Online Datasheet in PDF
Product Name
TIGIT, Monoclonal Antibody
Full Product Name
TIGIT Antibody [4A12]
Product Gene Name
anti-TIGIT antibody
Product Synonym Names
T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; WUCAM
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4A12
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid
Supplied in PBS containing 0.02% sodium azide.
Purity/Purification
Protein A purified
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 26 kDa
Observed: 47 kDa
OMIM
612859
3D Structure
ModBase 3D Structure for Q495A1
Immunogen
TIGIT antibody was raised against the extracellular domain of human TIGIT
Conjugate
Unconjugated

Product Categories/Family for anti-TIGIT antibody

Immunology
Applications Tested/Suitable for anti-TIGIT antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-TIGIT antibody
IHC starting at 2 ug/mL
IF start at 1 ug/mL
FC/FACS at 1 ug/ml
ICC at 1 ug/mL
WB at 1 ug/mL

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Western Blot (WB) of anti-TIGIT antibody

Western blot analysis of TIGIT in over expressing HEK293 cells using antibody at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml.
Mouse Anti-TIGIT Monoclonal Antibody – Cat# MBS154593

Immunocytochemistry (IHC) of anti-TIGIT antibody

Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Mouse Anti-TIGIT Monoclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS154593

Immunofluorescence (IF) of anti-TIGIT antibody

Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml.

Green: TIGIT Antibody [4A12]
Blue: DAPI staining
Mouse Anti-TIGIT Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154593

Immunofluorescence (IF) of anti-TIGIT antibody

Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml.

Green: TIGIT Antibody [4A12]
Blue: DAPI staining
Mouse Anti-TIGIT Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154593

Immunohistochemistry (IHC) of anti-TIGIT antibody

Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.
Mouse Anti-TIGIT Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154593

Flow Cytometry (FC/FACS) of anti-TIGIT antibody

Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.
Mouse Anti-TIGIT Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS154593

ELISA (EIA) of anti-TIGIT antibody

Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA at decreasing concentrations.
Mouse Anti-TIGIT Monoclonal Antibody, ELISA (EIA) – Cat# MBS154593

Product References and Citations for anti-TIGIT antibody

Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63. Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.

Other Notes

Small volumes of anti-TIGIT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TIGIT purchase

MBS154593 is an antibody targeting TIGIT. Also known as TIGIT Antibody [4A12]. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TIGIT. It may not necessarily be applicable to this product.

NCBI GI #
256600228
NCBI GeneID
201633
NCBI Accession #
NP_776160
UniProt Primary Accession #
Q495A1
UniProt Secondary Accession #
Q495A3; Q5JPD8; Q6MZS2; Q8N877
UniProt Related Accession #
Q495A1
GenBank Nucleotide #
NM_173799.3
NCBI Official Full Name
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Synonym Full Names
T cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol
TIGIT
NCBI Official Synonym Symbols
VSIG9; VSTM3; WUCAM
UniProt Protein Name
T-cell immunoreceptor with Ig and ITIM domains
UniProt Synonym Protein Names
V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain-containing protein 3
Protein Family
T-cell immunoreceptor with Ig and ITIM domains
UniProt Gene Name
TIGIT
UniProt Synonym Gene Names
VSIG9; VSTM3
NCBI Protein Information
T-cell immunoreceptor with Ig and ITIM domains

NCBI Summary for TIGIT

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]
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UniProt Comments for TIGIT

Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Research Articles on TIGIT

1. Natural killer cells and cytotoxic T cells express both TIGIT and DNAM-1 receptors, and in certain cases their effector functions are dictated by TIGIT or DNAM-1 signaling. Agonist and antagonist antibodies targeting either TIGIT or DNAM-1 present many therapeutic options for diseases spanning from cancer to auto-immunity. (Review)
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TIGIT antibody

 Reference Product  PubMed Publications
 CD226 antibody  >40 publications with TIGIT and CD226
 FOXP3 antibody  >22 publications with TIGIT and FOXP3
 CD96 antibody  >18 publications with TIGIT and CD96
 PVR antibody  >17 publications with TIGIT and PVR
 HAVCR2 antibody  >7 publications with TIGIT and HAVCR2
 HBZ antibody  >3 publications with TIGIT and HBZ
 PVRL2 antibody  >3 publications with TIGIT and PVRL2
 IL2RA antibody  >1 publications with TIGIT and IL2RA
 PVRL3 antibody  >1 publications with TIGIT and PVRL3

Pathways associated with anti-TIGIT antibody

 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram

Diseases associated with anti-TIGIT antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >20 publications with TIGIT and Inflammation
 Immunologic Deficiency Syndromes Antibodies  >5 publications with TIGIT and Immunologic Deficiency Syndromes
 Lung Neoplasms Antibodies  >5 publications with TIGIT and Lung Neoplasms
 Breast Neoplasms Antibodies  >4 publications with TIGIT and Breast Neoplasms
 Pregnancy Complications Antibodies  >2 publications with TIGIT and Pregnancy Complications
 Infant Death Antibodies  >1 publications with TIGIT and Infant Death
 Chromosome Disorders Antibodies  >1 publications with TIGIT and Chromosome Disorders
 Carcinoma, Hepatocellular Antibodies  >1 publications with TIGIT and Carcinoma, Hepatocellular
 Necrosis Antibodies  >1 publications with TIGIT and Necrosis

Organs/Tissues associated with anti-TIGIT antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >118 publications with TIGIT and Blood
 Lymph Node Antibodies  >10 publications with TIGIT and Lymph Node
 Lung Antibodies  >8 publications with TIGIT and Lung
 Spleen Antibodies  >7 publications with TIGIT and Spleen
 Kidney Antibodies  >1 publications with TIGIT and Kidney
 Thymus Antibodies  >1 publications with TIGIT and Thymus
 Stomach Antibodies  >1 publications with TIGIT and Stomach
 Brain Antibodies  >1 publications with TIGIT and Brain
 Mouth Antibodies  >1 publications with TIGIT and Mouth
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