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VISTA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154604

VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
VISTA, Monoclonal Antibody
Full Product Name
VISTA Antibody [6D2]
Product Gene Name
anti-VISTA antibody
Product Synonym Gene Name
VSIR
Product Synonym Names
VISTA molecule; VSIR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; VISTA; C10orf54; chromosome 10 open reading frame 54; PD-1H; V-set immunoregulatory receptor; V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation; Chromosome 10 Open Reading Frame 54
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D2
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid
Supplied in PBS containing 0.02% sodium azide.
Purity/Purification
Protein A purified
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 31 kDa
Observed: 47 kDa
OMIM
615608
3D Structure
ModBase 3D Structure for Q9H7M9
Immunogen
VISTA antibody was raised against the extracellular domain of human VISTA.
Conjugate
Unconjugated

Product Categories/Family for anti-VISTA antibody

Immunology
Applications Tested/Suitable for anti-VISTA antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-VISTA antibody
IHC starting at 5 ug/mL
ICC starting at 1 ug/mL
IF start at 5 ug/mL
FC/FACS at 10 ug/ml.

Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Immunocytochemistry (IHC) of anti-VISTA antibody

Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Mouse Anti-VISTA Monoclonal Antibody – Cat# MBS154604

Immunofluorescence (IF) of anti-VISTA antibody

Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL.

Green: VISTA Antibody [6D2]
Blue: DAPI staining
Mouse Anti-VISTA Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154604

Immunofluorescence (IF) of anti-VISTA antibody

Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 10 μg/mL.

Red: VISTA Antibody [6D2]
Blue: DAPI staining
Mouse Anti-VISTA Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154604

Immunofluorescence (IF) of anti-VISTA antibody

Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL.

Red: VISTA Antibody [6D2]
Blue: DAPI staining
Mouse Anti-VISTA Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154604

Immunohistochemistry (IHC) of anti-VISTA antibody

Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.
Mouse Anti-VISTA Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154604

Flow Cytometry (FC/FACS) of anti-VISTA antibody

Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.
Mouse Anti-VISTA Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS154604

ELISA (EIA) of anti-VISTA antibody

Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.
Mouse Anti-VISTA Monoclonal Antibody, ELISA (EIA) – Cat# MBS154604

Product References and Citations for anti-VISTA antibody

Mayya V., et al. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46. Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.

Other Notes

Small volumes of anti-VISTA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VISTA purchase

MBS154604 is an antibody targeting VISTA. Also known as VISTA Antibody [6D2]. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for VISTA. It may not necessarily be applicable to this product.

NCBI GI #
62339432
NCBI GeneID
64115
NCBI Accession #
NP_071436
UniProt Primary Accession #
Q9H7M9
UniProt Secondary Accession #
Q6UXF3; Q8WUG3; Q8WYZ8; A1L0X9; A4ZYV1; A8MVH5
UniProt Related Accession #
Q9H7M9
GenBank Nucleotide #
NM_022153.1
NCBI Official Full Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
NCBI Official Synonym Full Names
V-set immunoregulatory receptor
NCBI Official Symbol
VSIR
NCBI Official Synonym Symbols
B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
UniProt Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Synonym Protein Names
Platelet receptor Gi24
UniProt Gene Name
VSIR
UniProt Synonym Gene Names
Sisp-1
NCBI Protein Information
V-type immunoglobulin domain-containing suppressor of T-cell activation

UniProt Comments for VISTA

Immunoregulatory receptor which inhibits the T-cell response (PubMed:24691993). May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling (). May stimulate MMP14-mediated MMP2 activation (PubMed:20666777).

Research Articles on VISTA

1. Taken together, the results indicated that the VISTA high and CD8 low group, as an immunosuppressive subgroup, might be associated with a poor prognosis in primary OSCC. These findings indicated that VISTA might be a potential immunotherapeutic target in OSCC treatment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-VISTA antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1 publications with VISTA and Inflammation

Organs/Tissues associated with anti-VISTA antibody

 Organ/Tissue Name  Pubmed Publications
 Mouth Antibodies  >1 publications with VISTA and Mouth
 Blood Antibodies  >1 publications with VISTA and Blood
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