• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

LAG3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154617

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LAG3, Monoclonal Antibody
Full Product Name
LAG3 Antibody [1G4]
Product Gene Name
anti-LAG3 antibody
Product Synonym Names
lymphocyte activating 3; LAG-3; CD223
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1G4
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid
Supplied in PBS containing 0.02% sodium azide.
Purity/Purification
Protein A purified
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 55 kDa
Observed: 60; 70; 80 kDa
OMIM
153337
3D Structure
ModBase 3D Structure for P18627
Immunogen
LAG-3 antibody was raised against the extracellular domain of human LAG-3
Conjugate
Unconjugated

Product Categories/Family for anti-LAG3 antibody

Immunology
Applications Tested/Suitable for anti-LAG3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-LAG3 antibody
ELISA starting at 0.25 ug/mL
WB start at 0.25 ug/mL
ICC start at 1 ug/mL
IF start at 10 ug/mL
IHC start at 5 ug/mL
FC/FACS start at 1 ug/ml.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Western Blot (WB) of anti-LAG3 antibody

Western blot analysis of LAG-3 in over expressing HEK293 cells using LAG-3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.
Mouse Anti-LAG3 Monoclonal Antibody – Cat# MBS154617

Immunocytochemistry (IHC) of anti-LAG3 antibody

Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Mouse Anti-LAG3 Monoclonal Antibody, Immunocytochemistry (IHC) – Cat# MBS154617

Immunofluorescence (IF) of anti-LAG3 antibody

Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml.

Green: LAG3 Antibody [1G4]
Blue: DAPI staining
Mouse Anti-LAG3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154617

Immunofluorescence (IF) of anti-LAG3 antibody

Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml.

Green: LAG3 Antibody [1G4]
Blue: DAPI staining
Mouse Anti-LAG3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154617

Immunohistochemistry (IHC) of anti-LAG3 antibody

Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.
Mouse Anti-LAG3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154617

Flow Cytometry (FC/FACS) of anti-LAG3 antibody

Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.
Mouse Anti-LAG3 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS154617

ELISA (EIA) of anti-LAG3 antibody

Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA at decreasing concentrations.
Mouse Anti-LAG3 Monoclonal Antibody, ELISA (EIA) – Cat# MBS154617

ELISA (EIA) of anti-LAG3 antibody

A sandwich ELISA was performed using the anti-LAG3 mAbs as the capture antibodies for the LAG3 extracellular domain antigen with biotin-labeled Risk-Free anti-LAG3 mAbs as the detection antibodies.
Mouse Anti-LAG3 Monoclonal Antibody, ELISA (EIA) – Cat# MBS154617

Product References and Citations for anti-LAG3 antibody

Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216-21. Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619-22. Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885-91. Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970-9.

Other Notes

Small volumes of anti-LAG3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LAG3 purchase

MBS154617 is an antibody targeting LAG3. Also known as LAG3 Antibody [1G4]. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LAG3. It may not necessarily be applicable to this product.

NCBI GI #
167614500
NCBI GeneID
3902
NCBI Accession #
NP_002277
UniProt Primary Accession #
P18627
UniProt Secondary Accession #
Q7Z643; A8K7T9
UniProt Related Accession #
P18627
GenBank Nucleotide #
NM_002286.5
NCBI Official Full Name
lymphocyte activation gene 3 protein
NCBI Official Synonym Full Names
lymphocyte activating 3
NCBI Official Symbol
LAG3
NCBI Official Synonym Symbols
CD223
UniProt Protein Name
Lymphocyte activation gene 3 protein
UniProt Synonym Protein Names
Protein FDC; CD_antigen: CD223
Protein Family
Lymphocyte activation gene 3 protein
UniProt Gene Name
LAG3
UniProt Synonym Gene Names
FDC; LAG-3
NCBI Protein Information
lymphocyte activation gene 3 protein

NCBI Summary for LAG3

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

UniProt Comments for LAG3

Involved in lymphocyte activation. Binds to HLA class-II antigens.

Research Articles on LAG3

1. Among the 89 patients, CD274, LAG3, and IDO1 expressions in TIICs were observed in 68.6% (61 cases), 13.5% (12), and 28.1% (25) of patients, respectively. CD274, CTLA4, and IDO1 were expressed in tumor cells of 24.7% (22 cases), 4.5% (4), and 72.0% (64) of patients, respectively.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LAG3 antibody

 Reference Product  PubMed Publications
 FOXP3 antibody  >42 publications with LAG3 and FOXP3
 PDCD1 antibody  >33 publications with LAG3 and PDCD1
 CTLA4 antibody  >33 publications with LAG3 and CTLA4
 CD274 antibody  >18 publications with LAG3 and CD274
 HAVCR2 antibody  >9 publications with LAG3 and HAVCR2
 CD160 antibody  >6 publications with LAG3 and CD160
 GZMB antibody  >5 publications with LAG3 and GZMB
 TBX21 antibody  >3 publications with LAG3 and TBX21
 TNFRSF18 antibody  >2 publications with LAG3 and TNFRSF18

Pathways associated with anti-LAG3 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 MHC Class II Antigen Presentation Pathway antibodies  MHC Class II Antigen Presentation Pathway Diagram

Diseases associated with anti-LAG3 antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >50 publications with LAG3 and Death
 Inflammation Antibodies  >27 publications with LAG3 and Inflammation
 Carcinoma Antibodies  >19 publications with LAG3 and Carcinoma
 Lung Neoplasms Antibodies  >9 publications with LAG3 and Lung Neoplasms
 Necrosis Antibodies  >9 publications with LAG3 and Necrosis
 Neoplasms, Experimental Antibodies  >7 publications with LAG3 and Neoplasms, Experimental
 Carcinoma, Squamous Cell Antibodies  >6 publications with LAG3 and Carcinoma, Squamous Cell
 Liver Diseases Antibodies  >6 publications with LAG3 and Liver Diseases
 Adenocarcinoma Antibodies  >5 publications with LAG3 and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >4 publications with LAG3 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-LAG3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >118 publications with LAG3 and Blood
 Spleen Antibodies  >14 publications with LAG3 and Spleen
 Brain Antibodies  >8 publications with LAG3 and Brain
 Intestine Antibodies  >6 publications with LAG3 and Intestine
 Testis Antibodies  >2 publications with LAG3 and Testis
 Uterus Antibodies  >2 publications with LAG3 and Uterus
 Prostate Antibodies  >1 publications with LAG3 and Prostate
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000