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B7-H3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154623

CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has… More details

$530 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
B7-H3, Monoclonal Antibody
Full Product Name
B7-H3 Antibody [10G6]
Product Gene Name
Product Synonym Gene Name
CD276
Product Synonym Names
CD276 molecule; B7H3; B7RP-2; 4Ig-B7-H3; CD276
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
10G6
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid
Supplied in PBS containing 0.02% sodium azide.
Purity/Purification
Protein A purified
Concentration
1 mg/mL (lot specific)
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
57,165 Da
OMIM
605715
3D Structure
ModBase 3D Structure for Q5ZPR3
Immunogen
B7-H3 antibody was raised against the extracellular domain of human B7-H3
Conjugate
Unconjugated

Product Categories/Family for anti-B7-H3 antibody

Applications Tested/Suitable for anti-B7-H3 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-B7-H3 antibody
IHC starting at 2 ug/mL
ICC starting at 1 ug/mL
IF start at 10 ug/mL
FC/FACS at 1 ug/ml.

Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Immunocytochemistry (IHC) of anti-B7-H3 antibody

Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.
Mouse Anti-B7-H3 Monoclonal Antibody – Cat# MBS154623

Immunofluorescence (IF) of anti-B7-H3 antibody

Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.

Green: B7-H3 Antibody [10G6]
Blue: DAPI staining
Mouse Anti-B7-H3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154623

Immunofluorescence (IF) of anti-B7-H3 antibody

Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml.

Green: B7-H3 Antibody [10G6]
Blue: DAPI staining
Mouse Anti-B7-H3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154623

Immunohistochemistry (IHC) of anti-B7-H3 antibody

Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.
Mouse Anti-B7-H3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154623

Flow Cytometry (FC/FACS) of anti-B7-H3 antibody

Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.
Mouse Anti-B7-H3 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS154623

ELISA (EIA) of anti-B7-H3 antibody

Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA at decreasing concentrations.
Mouse Anti-B7-H3 Monoclonal Antibody, ELISA (EIA) – Cat# MBS154623

Product References and Citations for anti-B7-H3 antibody

Chapoval AI, Ni J, Lau JS, et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol 2001; 2:269-74. Sun M, Richards S, Prasad DV, et al. Characterization of mouse and human B7-H3 genes. J Immunol 2002; 168:6294-7. Yi KH and Chen L. Fine tuning the immune response through B7-H3 and B7-H4. Immunol Rev 2009; 229:145-51. Wang L, Kang FB, and Shan BE. B7-H3-mediated tumor immunology: friend or foe. Int J Cancer 2014; 134:2764-71.

Other Notes

Small volumes of anti-B7-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for B7-H3 purchase

MBS154623 is an antibody targeting B7-H3. Also known as B7-H3 Antibody [10G6]. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for B7-H3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD276 antigen isoform a
NCBI Official Synonym Full Names
CD276 molecule
NCBI Official Symbol
CD276
NCBI Official Synonym Symbols
B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
UniProt Protein Name
CD276 antigen
UniProt Synonym Protein Names
4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD_antigen: CD276
UniProt Gene Name
CD276
UniProt Synonym Gene Names
B7H3; B7-H3
NCBI Protein Information
CD276 antigen

NCBI Summary for B7-H3

The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
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UniProt Comments for B7-H3

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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