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PDL1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS154685

PD-L1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells… More details

$530 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
PDL1 [5H6], Monoclonal Antibody
Full Product Name
PDL1 Antibody [5H6]
Product Gene Name
anti-PDL1 antibody
Product Synonym Gene Name
CD274
Product Synonym Names
programmed cell death 1 ligand; PD-L1 Antibody: Programmed cell death 1 ligand-1; programmed death ligand 1; PDL1; PDL-1; B7-H1
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5H6
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid
PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Purity/Purification
PD-L1 Antibody is supplied as protein A purified IgG1
Concentration
1mg/mL (lot specific)
Preparation and Storage
PD-L1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Molecular Weight
Predicted: 33kDa
Observed: 37kDa
OMIM
605402
3D Structure
ModBase 3D Structure for Q9NZQ7
Source
Human
Immunogen
PD-L1 antibody was raised against the extracellular domain of human PD-L1.
Conjugation
Unconjugated

Product Categories/Family for anti-PDL1 antibody

Immunology
Applications Tested/Suitable for anti-PDL1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)-Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-PDL1 antibody
PD-L1 antibody can be used for detection of PD-L1 by Western blot at 0.5-1ug/mL. Antibody can also be used for immunohistochemistry starting at 2-5ug/mL. For immunofluorescence start at 5ug/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Immunocytochemistry (CC) of anti-PDL1 antibody

Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.
Mouse Anti-PDL1 Monoclonal Antibody – Cat# MBS154685

Immunofluorescence (IF) of anti-PDL1 antibody

Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 μg/mL.
Red: PDL1 Antibody [1D7] (RF16033)
Blue: DAPI staining
Mouse Anti-PDL1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154685

Immunofluorescence (IF) of anti-PDL1 antibody

Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 μg/mL.
Red: PDL1 Antibody [1D7] (RF16033)
Blue: DAPI staining
Mouse Anti-PDL1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS154685

Immunohistochemistry (IHC) of anti-PDL1 antibody

Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 10 μg/mL.
Mouse Anti-PDL1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS154685

Flow Cytometry (FC/FACS) of anti-PDL1 antibody

Flow cytometry analysis of PD-L1 overexpressing HEK293 cells using PD-L1 antibody and control mouse IgG antibody at 0.1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L1 overexpressing HEK293 cells.
Mouse Anti-PDL1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS154685

Product References and Citations for anti-PDL1 antibody

Holling , Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
Aydin AM, Woldu SL, Hutchinson RC, et al. Spotlight on atezolizumab and its potential in the treaent of advanced urothelial bladder cancer.Onco. Targets Ther. 2017;10:1487-502.

Other Notes

Small volumes of anti-PDL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDL1 purchase

MBS154685 is an antibody targeting PDL1. Also known as PDL1 Antibody [5H6]. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PDL1. It may not necessarily be applicable to this product.

NCBI GI #
7661534
NCBI GeneID
29126
NCBI Accession #
NP_054862
UniProt Primary Accession #
Q9NZQ7
UniProt Secondary Accession #
Q14CJ2; Q2V8D5; Q66RK1; Q6WEX4; Q9NUZ5; B2RBA2; B4DU27
UniProt Related Accession #
Q9NZQ7
GenBank Nucleotide #
NM_014143.3
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform a
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Synonym Protein Names
B7 homolog 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1

UniProt Comments for PDL1

CD274: Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: plasma membrane; integral to membrane; endomembrane system; external side of plasma membrane

Molecular Function: protein binding

Biological Process: regulation of activated T cell proliferation; cell surface receptor linked signal transduction; negative regulation of interleukin-10 production; negative regulation of interferon-gamma production; negative regulation of activated T cell proliferation; T cell costimulation; immune response; positive regulation of T cell proliferation; signal transduction
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Research Articles on PDL1

1. The recent development of the checkpoint inhibitors anti-programmed death-1 and anti-programmed death ligand-1 has generated an enormous amount of interest as a potential new anticancer strategy in solid tumors

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PDL1 antibody

 Reference Product  PubMed Publications
 CD80 antibody  >391 publications with PDL1 and CD80
 PDCD1 antibody  >332 publications with PDL1 and PDCD1
 STAT1 antibody  >12 publications with PDL1 and STAT1
 PTEN antibody  >10 publications with PDL1 and PTEN

Pathways associated with anti-PDL1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 PD-1 Signaling Pathway antibodies  PD-1 Signaling Pathway Diagram

Diseases associated with anti-PDL1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >109 publications with PDL1 and Inflammation
 Liver Diseases Antibodies  >86 publications with PDL1 and Liver Diseases
 Necrosis Antibodies  >74 publications with PDL1 and Necrosis
 Nervous System Diseases Antibodies  >70 publications with PDL1 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >69 publications with PDL1 and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >64 publications with PDL1 and Adenocarcinoma
 Lung Diseases Antibodies  >46 publications with PDL1 and Lung Diseases
 Kidney Diseases Antibodies  >44 publications with PDL1 and Kidney Diseases
 Lung Neoplasms Antibodies  >44 publications with PDL1 and Lung Neoplasms
 Recurrence Antibodies  >26 publications with PDL1 and Recurrence

Organs/Tissues associated with anti-PDL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lymph Node Antibodies  >79 publications with PDL1 and Lymph Node
 Lung Antibodies  >73 publications with PDL1 and Lung
 Bone Antibodies  >62 publications with PDL1 and Bone
 Skin Antibodies  >45 publications with PDL1 and Skin
 Brain Antibodies  >31 publications with PDL1 and Brain
 Pancreas Antibodies  >28 publications with PDL1 and Pancreas
 Placenta Antibodies  >19 publications with PDL1 and Placenta
 Stomach Antibodies  >13 publications with PDL1 and Stomach
 Mouth Antibodies  >9 publications with PDL1 and Mouth
 Prostate Antibodies  >7 publications with PDL1 and Prostate
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