Figure 1 Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: hRIP3, 8963 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 2 Western Blot Validation in Human Tissues
Loading: 15 μg of lysates per lane.
Antibodies: hRIP3, 8963 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 3 Western Blot Validation of hRIP3 in Human THP-1 Cell Lysate
Loading: 15 μg of lysates per lane.
Antibodies: hRIP3, 8963 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Lane 1-2: human THP-1 cell lysate in the absence (Lane 1) or the presence (Lane 2) of blocking peptide.
Figure 4 Immunofluorescence Validation of hRIP3 in Molt4 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling hRIP3 with 8963 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 5 Immunofluorescence Validation of hRIP3 in Human Breast Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human breast tissue labeling hRIP3 with 8963 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 6 Immunofluorescence Validation of hRIP3 in Human Colon Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human colon tissue labeling hRIP3 with 8963 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 7 Immunohistochemistry Validation of hRIP3 in Human Liver Tissue
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-hRIP3 antibody (8963) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 8 Immunohistochemistry Validation of hRIP3 in Human Breast Tissue
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-hRIP3 antibody (8963) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 9 Immunohistochemistry Validation of hRIP3 in Human Colon Tissue
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-hRIP3 antibody (8963) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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