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Bone Sialoprotein, BSP (BSP) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS160069

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Bone Sialoprotein (also known as BSP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.Principles of the Assay: This kit is an Enzyme-Linked… More details

$445 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
bone sialoprotein, BSP, ELISA Kit
Full Product Name
Mouse bone sialoprotein, BSP ELISA Kit
Product Gene Name
Species Reactivity
Mouse
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Detection Range
0.05ng/ml - 20ng/ml
Sensitivity
0.028ng/ml
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Molecular Weight
35,148 Da
3D Structure
ModBase 3D Structure for P21815
Intra-Assay
CV<8%
Inter-Assay
CV<10%
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of BSP elisa kit

Mouse BSP ELISA Kit – Cat# MBS160069

Other Notes

Small volumes of BSP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BSP purchase

MBS160069 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the bone sialoprotein, BSP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BSP. The ELISA analytical biochemical technique of the MBS160069 kit is based on BSP antibody-BSP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BSP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BSP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for BSP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
NCBI Official Full Name
bone sialoprotein
NCBI Official Synonym Full Names
integrin binding sialoprotein
NCBI Official Symbol
IBSP
NCBI Official Synonym Symbols
BSP; BNSP; SP-II; BSP-II
UniProt Protein Name
Bone sialoprotein 2
UniProt Synonym Protein Names
Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein
Protein Family
UniProt Gene Name
IBSP
UniProt Synonym Gene Names
BNSP; BSP II
NCBI Protein Information
bone sialoprotein 2

NCBI Summary for BSP

The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]
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UniProt Comments for BSP

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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