5-Hydroxyindolacetic Acid (5-HIAA) ELISA Kit for Rat

5-Hydroxyindoleacetic acid (5-HIAA) is the main metabolite of serotonin in the human body. In chemical analysis of urine samples, 5-HIAA is used to determine the body's levels of serotonin.
5-HIAA is tested by 24-hour urine samples combined with an acidic additive to maintain pH below 3. Certain foods and drugs are known to interfere with the measurement, and levels can vary depending on complications, such as a tumor, renal malfunction, small bowel resection, among other things. Since 5-HIAA is a metabolite of serotonin, testing is most frequently performed for the identification of carcinoid tumors of the enterochromaffin (Kultschitzsky) cells of the small intestine, which release large amounts of serotonin. Values greater than 25 mg per 24 hours (higher if the patient has malabsorption) are strong evidence for carcinoid.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate 5-HIAA in samples. An antibody specific for 5-HIAA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any5-HIAA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-HIAA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-HIAA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of 5-HIAA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281460 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxyindolacetic acid (5-HIAA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 5-HIAA. The ELISA analytical biochemical technique of the MBS281460 kit is based on 5-HIAA antibody-5-HIAA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5-HIAA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5-HIAA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).