Tyrosine Kinase with Immunoglobulin-Like and EGF-Like Domains 2, Tie-2 (TIE-2) ELISA Kit for Rat

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Tyrosine Kinase with Immunoglobulin-like and (also known as Tie-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat Tie-2 antibody. Tie-2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Tie-2 Antibody is added and binds to Tie-2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Tie-2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat Tie-2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of Tie-2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS161013 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the tyrosine kinase with immunoglobulin-like and EGF-like domains 2, Tie-2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Tie-2. The ELISA analytical biochemical technique of the MBS161013 kit is based on Tie-2 antibody-Tie-2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Tie-2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Tie-2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).