Creatine Kinase, Ck (CK) ELISA Kit for Human

Name: Human CK ELISA Kit; Creatine Kinase, Ck; MyBioSource
Brand: MyBioSource
SKU: MBS161183
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS161183

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Creatine Kinase (also known as CK) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.Principles of the Assay: This kit is an Enzyme-Linked… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Creatine Kinase, CK, ELISA Kit

Full Product Name

Human Creatine Kinase, CK ELISA Kit

Product Gene Name

CK elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, cell lysates, tissue homogenates

Detection Range

5U/L - 1500U/L

Sensitivity

2.52U/L

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Molecular Weight

50,421 Da

3D Structure

ModBase 3D Structure for P12532

Intra-Assay

CV<8%

Inter-Assay

CV<10%

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CK purchase

MBS161183 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Creatine Kinase, CK, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CK. The ELISA analytical biochemical technique of the MBS161183 kit is based on CK antibody-CK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CK. It may not necessarily be applicable to this product.

NCBI GI #

180590

NCBI GeneID

1159

NCBI Accession #

AAA98744.1

UniProt Primary Accession #

P12532

UniProt Secondary Accession #

Q0VAM3; Q32NF6; Q53FC4; B4DIT8; B7ZA09

UniProt Related Accession #

P12532

NCBI Official Full Name

creatine kinase

NCBI Official Synonym Full Names

creatine kinase, mitochondrial 1B

NCBI Official Symbol

CKMT1B

NCBI Official Synonym Symbols

CKMT; CKMT1; UMTCK

UniProt Protein Name

Creatine kinase U-type, mitochondrial

UniProt Synonym Protein Names

Acidic-type mitochondrial creatine kinase; Mia-CK; Ubiquitous mitochondrial creatine kinase; U-MtCK

UniProt Gene Name

CKMT1A

UniProt Synonym Gene Names

CKMT; Mia-CK; U-MtCK

NCBI Protein Information

creatine kinase U-type, mitochondrial

NCBI Summary for CK

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]

UniProt Comments for CK

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Research Articles on CK

1. The highly conserved residue E227 acts as the catalytic base to accept the guanidinium proton transferred from creatine in UMTCK.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CK elisa kit

Reference Product	PubMed Publications
CATSPER2 elisa kit	>2 publications with CK and CATSPER2

Pathways associated with CK elisa kit

Products by Pathway	Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Creatine Metabolism Pathway antibodies	Creatine Metabolism Pathway Diagram
Creatine Pathway antibodies	Creatine Pathway Diagram
Creatine Pathway antibodies	Creatine Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Creatine-phosphate Biosynthesis Pathway antibodies	Creatine-phosphate Biosynthesis Pathway Diagram
Creatine-phosphate Biosynthesis Pathway antibodies	Creatine-phosphate Biosynthesis Pathway Diagram

Diseases associated with CK elisa kit

Disease Name	Pubmed Publications
Anemia Antibodies	>1 publications with CK and Anemia
Breast Neoplasms Antibodies	>1 publications with CK and Breast Neoplasms
Nervous System Diseases Antibodies	>1 publications with CK and Nervous System Diseases

Organs/Tissues associated with CK elisa kit

Organ/Tissue Name	Pubmed Publications
Lymph Antibodies	>1 publications with CK and Lymph