Prothrombin, Pt (PT) ELISA Kit for Human

Name: Human PT ELISA Kit; Prothrombin, Pt; MyBioSource
Brand: MyBioSource
SKU: MBS161529
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS161529

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PT antibody. PT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PT Antibody is… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Prothrombin, PT, ELISA Kit

Full Product Name

Human Prothrombin, PT ELISA Kit

Product Gene Name

PT elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids

Detection Range

5-2000U/L

Sensitivity

2.49U/L

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

70,037 Da

3D Structure

ModBase 3D Structure for P00734

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PT purchase

MBS161529 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Prothrombin, PT, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PT. The ELISA analytical biochemical technique of the MBS161529 kit is based on PT antibody-PT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PT. It may not necessarily be applicable to this product.

NCBI GI #

261695

NCBI GeneID

2147

NCBI Accession #

AAB24476.1

UniProt Primary Accession #

P00734

UniProt Secondary Accession #

Q4QZ40; Q53H04; Q53H06; Q69EZ7; Q7Z7P3; Q9UCA1; B2R7F7; B4E1A7

UniProt Related Accession #

P00734

NCBI Official Full Name

prothrombin, partial

NCBI Official Synonym Full Names

coagulation factor II, thrombin

NCBI Official Symbol

NCBI Official Synonym Symbols

PT; THPH1; RPRGL2

UniProt Protein Name

Prothrombin

UniProt Synonym Protein Names

Coagulation factor II

UniProt Gene Name

NCBI Protein Information

prothrombin

NCBI Summary for PT

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

UniProt Comments for PT

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Research Articles on PT

1. digestion of thrombin by P. aeruginosa elastase leads to the release of the C-terminal thrombin-derived peptide FYT21, which inhibits pro-inflammatory responses to several pathogen-associated molecular patterns.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PT elisa kit

Reference Product	PubMed Publications
FGA elisa kit	>8 publications with PT and FGA
FGB elisa kit	>6 publications with PT and FGB
FGG elisa kit	>5 publications with PT and FGG
F2R elisa kit	>5 publications with PT and F2R
GP1BA elisa kit	>5 publications with PT and GP1BA
THBD elisa kit	>2 publications with PT and THBD
SERPINC1 elisa kit	>1 publications with PT and SERPINC1

Pathways associated with PT elisa kit

Products by Pathway	Pathway Diagram
Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies	Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
Blood Clotting Cascade Pathway antibodies	Blood Clotting Cascade Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Common Pathway antibodies	Common Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies	FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram

Diseases associated with PT elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>1031 publications with PT and Nervous System Diseases
Liver Diseases Antibodies	>700 publications with PT and Liver Diseases
Neoplasms Antibodies	>677 publications with PT and Neoplasms
Hypertension Antibodies	>658 publications with PT and Hypertension
Drug Toxicity Antibodies	>614 publications with PT and Drug Toxicity
Inflammation Antibodies	>523 publications with PT and Inflammation
Liver Cirrhosis Antibodies	>465 publications with PT and Liver Cirrhosis
Brain Diseases Antibodies	>454 publications with PT and Brain Diseases
Heart Diseases Antibodies	>297 publications with PT and Heart Diseases
Kidney Diseases Antibodies	>256 publications with PT and Kidney Diseases

Organs/Tissues associated with PT elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1281 publications with PT and Liver
Uterus Antibodies	>955 publications with PT and Uterus
Kidney Antibodies	>718 publications with PT and Kidney
Heart Antibodies	>678 publications with PT and Heart
Embryonic Tissue Antibodies	>365 publications with PT and Embryonic Tissue
Connective Tissue Antibodies	>251 publications with PT and Connective Tissue
Spleen Antibodies	>229 publications with PT and Spleen
Bone Marrow Antibodies	>104 publications with PT and Bone Marrow
Mammary Gland Antibodies	>32 publications with PT and Mammary Gland
Uncharacterized Tissue Antibodies	>2 publications with PT and Uncharacterized Tissue