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Mothers Against Decapentaplegic Homolog 3 (SMAD3) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS161553

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Mothers Against Decapentaplegic Homolog 3 (also known as Smad3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.Principles of the Assay: This kit… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mothers Against Decapentaplegic Homolog 3 (Smad3), ELISA Kit
Full Product Name
Human Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit
Product Gene Name
Smad3 elisa kit
Species Reactivity
Human
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Detection Range
0.1ng/ml - 35ng/ml
Sensitivity
0.047ng/ml
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Molecular Weight
43,237 Da
3D Structure
ModBase 3D Structure for P84022
Intra-Assay
CV<8%
Inter-Assay
CV<10%
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of Smad3 elisa kit

Human SMAD3 ELISA Kit – Cat# MBS161553

Other Notes

Small volumes of Smad3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Smad3 purchase

MBS161553 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mothers Against Decapentaplegic Homolog 3 (Smad3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Smad3. The ELISA analytical biochemical technique of the MBS161553 kit is based on Smad3 antibody-Smad3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Smad3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Smad3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Smad3. It may not necessarily be applicable to this product.

NCBI GI #
18418623
NCBI GeneID
4088
NCBI Accession #
AAL68976.1
UniProt Primary Accession #
P84022
UniProt Secondary Accession #
O09064; O09144; O14510; O35273; Q92940; Q93002; A8K4B6; B7Z4Z5; B7Z6M9; B7Z9Q2; F5H383
UniProt Related Accession #
P84022
NCBI Official Full Name
Smad3
NCBI Official Synonym Full Names
SMAD family member 3
NCBI Official Symbol
SMAD3
NCBI Official Synonym Symbols
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
UniProt Protein Name
Mothers against decapentaplegic homolog 3
UniProt Synonym Protein Names
JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Protein Family
Mothers against decapentaplegic
UniProt Gene Name
SMAD3
UniProt Synonym Gene Names
MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3
NCBI Protein Information
mothers against decapentaplegic homolog 3

NCBI Summary for Smad3

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
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UniProt Comments for Smad3

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
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Research Articles on Smad3

1. The results suggest that co-expression of active SMAD2/3 could enhance multiple types of transcription factors (TF)-based cell identity conversion and therefore be a powerful tool for cellular engineering.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with Smad3 elisa kit

 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram

Diseases associated with Smad3 elisa kit

 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >692 publications with Smad3 and Fibrosis
 Neoplasms Antibodies  >592 publications with Smad3 and Neoplasms
 Inflammation Antibodies  >252 publications with Smad3 and Inflammation
 Disease Models, Animal Antibodies  >248 publications with Smad3 and Disease Models, Animal
 Liver Diseases Antibodies  >178 publications with Smad3 and Liver Diseases
 Kidney Diseases Antibodies  >155 publications with Smad3 and Kidney Diseases
 Cardiovascular Diseases Antibodies  >153 publications with Smad3 and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >130 publications with Smad3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >102 publications with Smad3 and Breast Neoplasms
 Necrosis Antibodies  >95 publications with Smad3 and Necrosis

Organs/Tissues associated with Smad3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >463 publications with Smad3 and Muscle
 Lung Antibodies  >280 publications with Smad3 and Lung
 Skin Antibodies  >200 publications with Smad3 and Skin
 Heart Antibodies  >146 publications with Smad3 and Heart
 Embryonic Tissue Antibodies  >142 publications with Smad3 and Embryonic Tissue
 Intestine Antibodies  >56 publications with Smad3 and Intestine
 Thyroid Antibodies  >28 publications with Smad3 and Thyroid
 Ascites Antibodies  >1 publications with Smad3 and Ascites
 Uncharacterized Tissue Antibodies  >1 publications with Smad3 and Uncharacterized Tissue
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