Beta2-Glycoprotein 1 Antibody IgA/G/M, Beta2-GP1 IgA/G/M (beta2-GP1 IgA/G/M) ELISA Kit for Rat

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of beta2-GP1 IgA/G/M elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS162451 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the beta2-glycoprotein 1 antibody IgA/G/M, beta2-GP1 IgA/G/M, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing beta2-GP1 IgA/G/M. The ELISA analytical biochemical technique of the MBS162451 kit is based on beta2-GP1 IgA/G/M antibody-beta2-GP1 IgA/G/M antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect beta2-GP1 IgA/G/M antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, beta2-GP1 IgA/G/M. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).