Coiled Coil Domain Containing Protein 80 (CCDC80) ELISA Kit for Rat

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Coiled-coil Domain-containing Protein 80 (also known as CCDC80) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CCDC80 antibody. CCDC80 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CCDC80 Antibody is added and binds to CCDC80 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CCDC80 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CCDC80. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of CCDC80 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS162975 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Coiled Coil Domain Containing Protein 80 (CCDC80) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CCDC80. The ELISA analytical biochemical technique of the MBS162975 kit is based on CCDC80 antibody-CCDC80 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CCDC80 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CCDC80. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).