5-Lipoxygenase (5-LO/LOX) ELISA Kit for Rat

Intended Uses: This LOX5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat LOX5. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: LOX5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LOX5 antibody and an LOX5-HRP conjugate. The assay sample and buffer are incubated together with LOX5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LOX5 concentration since LOX5 from samples and LOX5-HRP conjugate compete for the anti-LOX5 antibody binding site. Since the number of sites is limited, as more sites are occupied by LOX5 from the sample, fewer sites are left to bind LOX5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX5 concentration in each sample is interpolated from this standard curve.

Small volumes of 5-LO/LOX elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS722629 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-lipoxygenase (5-LO/LOX) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 5-LO/LOX. The ELISA analytical biochemical technique of the MBS722629 kit is based on 5-LO/LOX antibody-5-LO/LOX antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 5-LO/LOX antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 5-LO/LOX. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).