Wingless Type MMTV Integration Site Family, Member 10b (WNT10B) ELISA Kit for Human

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Wingless Type MMTV Integration Site Family, Member 10B (also known as WNT10B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human WNT10B antibody. WNT10B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human WNT10B Antibody is added and binds to WNT10B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT10B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human WNT10B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of WNT10B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS163831 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT10B. The ELISA analytical biochemical technique of the MBS163831 kit is based on WNT10B antibody-WNT10B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT10B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT10B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).