Lipase, Monoacylglycerol (MGL) ELISA Kit for Human

Name: Human MGL ELISA Kit; Lipase, Monoacylglycerol; MyBioSource
Brand: MyBioSource
SKU: MBS2024623
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2024623

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Monoacylglycerol (MGL). Standards or samples are then added to the appropriate microtiter… More details

$425 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Lipase, Monoacylglycerol (MGL), ELISA Kit

Full Product Name

Human Lipase, Monoacylglycerol (MGL) ELISA Kit

Product Gene Name

MGL elisa kit

Product Synonym Names

HU-K5, MGLL; MAGL; Monoglyceride Lipase; Lysophospholipase homolog; Lysophospholipase-like; Monoacylglycerol lipase

Species Reactivity

Human

Assay Type

Double-antibody Sandwich

Samples

Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.059ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Lipase, Monoacylglycerol (MGL).
No significant cross-reactivity or interference between Lipase, Monoacylglycerol (MGL) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

31,149 Da

OMIM

609699

3D Structure

ModBase 3D Structure for Q99685

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Lipase, Monoacylglycerol (MGL) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Lipase, Monoacylglycerol (MGL) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

Product Categories/Family for MGL elisa kit

Enzyme & Kinase

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of MGL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MGL purchase

MBS2024623 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lipase, Monoacylglycerol (MGL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MGL. The ELISA analytical biochemical technique of the MBS2024623 kit is based on MGL antibody-MGL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MGL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MGL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MGL. It may not necessarily be applicable to this product.

NCBI GI #

375268702

NCBI GeneID

11343

NCBI Accession #

NP_001243514.1

UniProt Primary Accession #

Q99685

UniProt Secondary Accession #

Q6IBG9; Q96AA5; B3KRC2; B7Z9D1

UniProt Related Accession #

Q99685

GenBank Nucleotide #

NM_001256585.1

NCBI Official Full Name

monoglyceride lipase isoform 3

NCBI Official Synonym Full Names

monoglyceride lipase

NCBI Official Symbol

MGLL

NCBI Official Synonym Symbols

MGL; HUK5; MAGL; HU-K5

UniProt Protein Name

Monoglyceride lipase

UniProt Synonym Protein Names

HU-K5

Protein Family

Methionine gamma-lyase

UniProt Gene Name

MGLL

UniProt Synonym Gene Names

MAGL

UniProt Entry Name

MGLL_HUMAN

NCBI Protein Information

monoglyceride lipase

NCBI Summary for MGL

This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]

UniProt Comments for MGL

MGLL: Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth. Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.

Protein type: Lipid Metabolism - glycerolipid; EC 3.1.1.23; Phospholipase

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; extrinsic to membrane; plasma membrane; synapse; cytosol

Molecular Function: protein homodimerization activity; acylglycerol lipase activity; lipid binding; lysophospholipase activity

Biological Process: platelet activation; glycerophospholipid biosynthetic process; regulation of signal transduction; regulation of axon extension; phospholipid metabolic process; regulation of inflammatory response; triacylglycerol catabolic process; arachidonic acid metabolic process; regulation of sensory perception of pain; lipid metabolic process; inflammatory response; blood coagulation; fatty acid biosynthetic process; acylglycerol catabolic process

Research Articles on MGL

1. Our findings establish that MAGL promotes metastases in nasopharyngeal carcinoma

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MGL elisa kit

Reference Product	PubMed Publications
PNPLA2 elisa kit	>3 publications with MGL and PNPLA2
LIPE elisa kit	>3 publications with MGL and LIPE
LPL elisa kit	>1 publications with MGL and LPL

Pathways associated with MGL elisa kit

Products by Pathway	Pathway Diagram
Acyl Chain Remodeling Of DAG And TAG Pathway antibodies	Acyl Chain Remodeling Of DAG And TAG Pathway Diagram
Acylglycerol Degradation Pathway antibodies	Acylglycerol Degradation Pathway Diagram
Acylglycerol Degradation Pathway antibodies	Acylglycerol Degradation Pathway Diagram
Arachidonate Production From DAG Pathway antibodies	Arachidonate Production From DAG Pathway Diagram
Effects Of PIP2 Hydrolysis Pathway antibodies	Effects Of PIP2 Hydrolysis Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram
Glycerolipid Metabolism Pathway antibodies	Glycerolipid Metabolism Pathway Diagram

Diseases associated with MGL elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>4 publications with MGL and Inflammation
Disease Models, Animal Antibodies	>3 publications with MGL and Disease Models, Animal
Fatty Liver Antibodies	>3 publications with MGL and Fatty Liver
Breast Neoplasms Antibodies	>2 publications with MGL and Breast Neoplasms
Liver Diseases Antibodies	>2 publications with MGL and Liver Diseases
Cardiovascular Diseases Antibodies	>1 publications with MGL and Cardiovascular Diseases
Weight Gain Antibodies	>1 publications with MGL and Weight Gain
Weight Loss Antibodies	>1 publications with MGL and Weight Loss
Prenatal Exposure Delayed Effects Antibodies	>1 publications with MGL and Prenatal Exposure Delayed Effects
Necrosis Antibodies	>1 publications with MGL and Necrosis

Organs/Tissues associated with MGL elisa kit

Organ/Tissue Name	Pubmed Publications
Adipose Tissue Antibodies	>5 publications with MGL and Adipose Tissue
Blood Antibodies	>5 publications with MGL and Blood
Liver Antibodies	>4 publications with MGL and Liver
Muscle Antibodies	>3 publications with MGL and Muscle
Intestine Antibodies	>3 publications with MGL and Intestine
Connective Tissue Antibodies	>2 publications with MGL and Connective Tissue
Brain Antibodies	>2 publications with MGL and Brain
Vascular Antibodies	>1 publications with MGL and Vascular
Skin Antibodies	>1 publications with MGL and Skin
Pancreas Antibodies	>1 publications with MGL and Pancreas