Cysteinyl Aspartate Specific Proteinases 3, Caspase-3/CPP32 (CASPASE-3/CPP32) ELISA Kit for Rat

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Cysteinyl Aspartate Specific Proteinases 3 (also known as CASPASE-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat CASPASE-3 antibody. CASPASE-3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CASPASE-3 Antibody is added and binds to CASPASE-3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CASPASE-3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat CASPASE-3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of Caspase-3/CPP32 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS164722 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cysteinyl aspartate specific proteinases 3, Caspase-3/CPP32, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Caspase-3/CPP32. The ELISA analytical biochemical technique of the MBS164722 kit is based on Caspase-3/CPP32 antibody-Caspase-3/CPP32 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Caspase-3/CPP32 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Caspase-3/CPP32. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).