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Anti-Thyroid-Peroxidase (TPO-Ab) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2801840

MBS2801840 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Anti-thyroid-Peroxidase antibody (TPO-Ab) ELISA Kit target analytes in biological samples. The concentration gradients… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Anti-thyroid-Peroxidase antibody (TPO-Ab), ELISA Kit
Full Product Name
Rat Anti-thyroid-Peroxidase antibody (TPO-Ab) ELISA Kit
Product Gene Name
Species Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat TPO-Ab. No significant cross-reactivity or interference between Rat TPO-Ab and analogues was observed.
Preparation and Storage
Store at 2-8 degree C
Asssay Type
Quantitive Competitive
Sample Types
Serum, Plasma, Other biological fluids
Detection Wavelength
450nm
Sample Volume
1-200uL
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
Test Principle
This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TPO-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated TPO-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled TPO-Ab and unlabeled TPO-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TPO-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Assay Duration
1-3h
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TPO-Ab elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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