Alkaline Phosphatase, ALP (ALP) ELISA Kit for Rat

Name: Rat ALP ELISA Kit; Alkaline Phosphatase, ALP; MyBioSource
Brand: MyBioSource
SKU: MBS165203
Price: 445 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS165203

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat ALP antibody. ALP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat ALP Antibody is… More details

$445 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Alkaline Phosphatase, ALP, ELISA Kit

Full Product Name

Rat Alkaline Phosphatase, ALP ELISA Kit

Product Gene Name

ALP elisa kit

Species Reactivity

Rat

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids

Detection Range

3-900U/L

Sensitivity

1.49U/L

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

48,909 Da

3D Structure

ModBase 3D Structure for P05186

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ALP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALP purchase

MBS165203 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alkaline Phosphatase, ALP, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALP. The ELISA analytical biochemical technique of the MBS165203 kit is based on ALP antibody-ALP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ALP. It may not necessarily be applicable to this product.

NCBI GI #

178462

NCBI GeneID

249

NCBI Accession #

AAB59378.1

UniProt Primary Accession #

P05186

UniProt Secondary Accession #

O75090; Q2TAI7; Q59EJ7; Q5BKZ5; Q5VTG5; Q6NZI8; Q8WU32; A1A4E7; B2RMP8; B7Z387; B7Z4Y6

UniProt Related Accession #

P05186

NCBI Official Full Name

alkaline phosphatase

NCBI Official Synonym Full Names

alkaline phosphatase, biomineralization associated

NCBI Official Symbol

ALPL

NCBI Official Synonym Symbols

HOPS; TNAP; TNALP; APTNAP; TNSALP; AP-TNAP

UniProt Protein Name

Alkaline phosphatase, tissue-nonspecific isozyme

UniProt Synonym Protein Names

Alkaline phosphatase liver/bone/kidney isozyme

Protein Family

Alkaline proteinase

UniProt Gene Name

ALPL

UniProt Synonym Gene Names

AP-TNAP; TNSALP

NCBI Protein Information

alkaline phosphatase, tissue-nonspecific isozyme

NCBI Summary for ALP

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]

UniProt Comments for ALP

This isozyme may play a role in skeletal mineralization.

Research Articles on ALP

1. genu varum is associated with the alkaline phosphatase level regardless of the presence of radiographic abnormalities in the growth plate in children.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ALP elisa kit

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram
Endochondral Ossification Pathway antibodies	Endochondral Ossification Pathway Diagram
Folate Biosynthesis Pathway antibodies	Folate Biosynthesis Pathway Diagram
Folate Biosynthesis Pathway antibodies	Folate Biosynthesis Pathway Diagram
TNF-alpha/NF-kB Signaling Pathway antibodies	TNF-alpha/NF-kB Signaling Pathway Diagram

Diseases associated with ALP elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>14 publications with ALP and Neoplasms
Kidney Diseases Antibodies	>9 publications with ALP and Kidney Diseases
Hypophosphatasia, Infantile Antibodies	>9 publications with ALP and Hypophosphatasia, Infantile
Hypophosphatasia, Childhood Antibodies	>8 publications with ALP and Hypophosphatasia, Childhood
Cardiovascular Diseases Antibodies	>8 publications with ALP and Cardiovascular Diseases
Seizures Antibodies	>7 publications with ALP and Seizures
Inflammation Antibodies	>6 publications with ALP and Inflammation
Pain Antibodies	>6 publications with ALP and Pain
Calcinosis Antibodies	>5 publications with ALP and Calcinosis
Hypertension Antibodies	>4 publications with ALP and Hypertension

Organs/Tissues associated with ALP elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>129 publications with ALP and Bone
Blood Antibodies	>43 publications with ALP and Blood
Liver Antibodies	>35 publications with ALP and Liver
Kidney Antibodies	>35 publications with ALP and Kidney
Connective Tissue Antibodies	>29 publications with ALP and Connective Tissue
Brain Antibodies	>12 publications with ALP and Brain
Mouth Antibodies	>11 publications with ALP and Mouth
Adipose Tissue Antibodies	>10 publications with ALP and Adipose Tissue
Embryonic Tissue Antibodies	>6 publications with ALP and Embryonic Tissue
Skin Antibodies	>6 publications with ALP and Skin