FMS-Like Tyrosine Kinase 3 Ligand, Flt-3l (FLT-3L) ELISA Kit for Mouse

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse FMS Like Tyrosine Kinase 3 Ligand (also known as Flt-3L) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse Flt-3L antibody. Flt-3L present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse Flt-3L Antibody is added and binds to Flt-3L in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Flt-3L antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse Flt-3L. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of Flt-3L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS165229 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the FMS-like tyrosine kinase 3 ligand, Flt-3L, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Flt-3L. The ELISA analytical biochemical technique of the MBS165229 kit is based on Flt-3L antibody-Flt-3L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Flt-3L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Flt-3L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).