Serum Amyloid A1 (SAA1) ELISA Kit for Bovine

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for SAA and Horseradish Peroxidase (HRP) conjugated SAA. The competitive inhibition reaction is launched between with HRP labeled SAA and unlabeled SAA with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of SAA in the sample. The color development is stopped and the intensity of the color is measured.

Metabolic load in dairy cows kept in herbage?based feeding systems and suitability of potential markers for compromised well?being. Zbinden, R. S., Falk, M., Münger, A., Dohme-Meier, F., van Dorland, H. A., Bruckmaier, R. M. and Gross, J. J. (2016)

Small volumes of SAA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS700691 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the serum amyloid A1 (SAA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SAA1. The ELISA analytical biochemical technique of the MBS700691 kit is based on SAA1 antibody-SAA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SAA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SAA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).