Neutrophil Elastase, Ne (NE) ELISA Kit for Canine

Name: Canine NE ELISA Kit; Neutrophil Elastase, Ne; MyBioSource
Brand: MyBioSource
SKU: MBS165823
Price: 575 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS165823

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine NE antibody. NE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine NE Antibody is… More details

$575 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Neutrophil elastase, NE, ELISA Kit

Full Product Name

Canine Neutrophil elastase, NE ELISA Kit

Product Gene Name

NE elisa kit

Species Reactivity

Canine

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids

Detection Range

0.5-100ng/ml

Sensitivity

0.24ng/ml

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

28,518 Da

3D Structure

ModBase 3D Structure for P08246

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NE purchase

MBS165823 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neutrophil elastase, NE, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NE. The ELISA analytical biochemical technique of the MBS165823 kit is based on NE antibody-NE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NE. It may not necessarily be applicable to this product.

NCBI GI #

386981

NCBI GeneID

1991

NCBI Accession #

AAA36359.1

UniProt Primary Accession #

P08246

UniProt Secondary Accession #

P09649; Q6B0D9; Q6LDP5

UniProt Related Accession #

P08246

NCBI Official Full Name

neutrophil elastase, partial

NCBI Official Synonym Full Names

elastase, neutrophil expressed

NCBI Official Symbol

ELANE

NCBI Official Synonym Symbols

GE; NE; HLE; HNE; ELA2; SCN1; PMN-E

UniProt Protein Name

Neutrophil elastase

UniProt Synonym Protein Names

Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase

UniProt Gene Name

ELANE

UniProt Synonym Gene Names

ELA2; HLE

NCBI Protein Information

neutrophil elastase

NCBI Summary for NE

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

UniProt Comments for NE

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.

Research Articles on NE

1. the effect of elastase on gammadelta T cells was mediated through the protease-activated receptor, PAR1, because the inhibition of this receptor with a specific antagonist, RWJ56110, abrogated the effect of neutrophils on gammadelta T-cell activation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NE elisa kit

Reference Product	PubMed Publications
MPO elisa kit	>1 publications with NE and MPO

Pathways associated with NE elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
Degradation Of Collagen Pathway antibodies	Degradation Of Collagen Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Systemic Lupus Erythematosus Pathway antibodies	Systemic Lupus Erythematosus Pathway Diagram
Systemic Lupus Erythematosus Pathway antibodies	Systemic Lupus Erythematosus Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Transcriptional Misregulation In Cancer Pathway antibodies	Transcriptional Misregulation In Cancer Pathway Diagram
Amb2 Integrin Signaling Pathway antibodies	Amb2 Integrin Signaling Pathway Diagram

Diseases associated with NE elisa kit

Disease Name	Pubmed Publications
Neutropenia Antibodies	>31 publications with NE and Neutropenia
Cyclic neutropenia Antibodies	>7 publications with NE and Cyclic neutropenia
NEUTROPENIA, SEVERE CONGENITAL, 1, AUTOSOMAL DOMINANT Antibodies	>5 publications with NE and NEUTROPENIA, SEVERE CONGENITAL, 1, AUTOSOMAL DOMINANT
Lung Diseases Antibodies	>3 publications with NE and Lung Diseases
Disease Models, Animal Antibodies	>2 publications with NE and Disease Models, Animal
Inflammation Antibodies	>2 publications with NE and Inflammation
Shock Antibodies	>1 publications with NE and Shock
Liver Diseases Antibodies	>1 publications with NE and Liver Diseases
Pain Antibodies	>1 publications with NE and Pain
Weight Loss Antibodies	>1 publications with NE and Weight Loss

Organs/Tissues associated with NE elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>18 publications with NE and Blood
Bone Marrow Antibodies	>5 publications with NE and Bone Marrow
Lung Antibodies	>3 publications with NE and Lung