Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit for Chicken

Intended Uses: This sandwich kit is for the accurate quantitative detection of Chicken Heat Shock 70kDa Protein 5 (also known as HSPA5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Chicken HSPA5 antibody. HSPA5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Chicken HSPA5 Antibody is added and binds to HSPA5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HSPA5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Chicken HSPA5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of HSPA5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS166061 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heat Shock 70kDa Protein 5 (HSPA5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HSPA5. The ELISA analytical biochemical technique of the MBS166061 kit is based on HSPA5 antibody-HSPA5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HSPA5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HSPA5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).