Breast Cancer Susceptibility Protein 1, BRCA-1 (BRCA-1) ELISA Kit for Rat

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Breast Cancer Susceptibility Protein 1 (also known as BRCA-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat BRCA-1 antibody. BRCA-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat BRCA-1 Antibody is added and binds to BRCA-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BRCA-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat BRCA-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of BRCA-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS166241 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Breast cancer susceptibility protein 1, BRCA-1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BRCA-1. The ELISA analytical biochemical technique of the MBS166241 kit is based on BRCA-1 antibody-BRCA-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BRCA-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BRCA-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).