Ki-67 Protein, Ki67p (ki67P) ELISA Kit for Human

Name: Human ki67P ELISA Kit; Ki-67 Protein, Ki67p; MyBioSource
Brand: MyBioSource
SKU: MBS166614
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS166614

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Ki-67 Protein (also known as Ki67P) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.Principles of the Assay: This kit is an Enzyme-Linked… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Ki-67 protein, ki67P, ELISA Kit

Full Product Name

Human Ki-67 protein, ki67P ELISA Kit

Product Gene Name

ki67P elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, cell lysates, tissue homogenates

Detection Range

0.1ng/ml - 40ng/ml

Sensitivity

0.056ng/ml

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Molecular Weight

324,718 Da

3D Structure

ModBase 3D Structure for Q61769

Intra-Assay

CV<8%

Inter-Assay

CV<10%

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ki67P elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ki67P purchase

MBS166614 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ki-67 protein, ki67P, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ki67P. The ELISA analytical biochemical technique of the MBS166614 kit is based on ki67P antibody-ki67P antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ki67P antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ki67P. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ki67P. It may not necessarily be applicable to this product.

NCBI GI #

1177528

NCBI GeneID

17345

NCBI Accession #

CAA58026.1

UniProt Primary Accession #

Q61769

UniProt Secondary Accession #

Q61769; Q7TSF6

UniProt Related Accession #

E9PVX6

NCBI Official Full Name

Ki-67

NCBI Official Synonym Full Names

antigen identified by monoclonal antibody Ki 67

NCBI Official Symbol

Mki67

NCBI Official Synonym Symbols

Ki67; Ki-67; D630048A14Rik

UniProt Protein Name

Proliferation marker protein Ki-67

UniProt Synonym Protein Names

Antigen identified by monoclonal antibody Ki-67 homologCurated; Antigen KI-67 homologCurated; Antigen Ki67 homologCurated

UniProt Gene Name

Mki67

UniProt Synonym Gene Names

Antigen KI-67 homologCurated; Antigen Ki67 homologCurated

NCBI Protein Information

proliferation marker protein Ki-67; antigen KI-67

UniProt Comments for ki67P

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.

Research Articles on ki67P

1. injection of a b3-adrenergic receptor (b3-AR) agonist for continuous 5 days increased the number of Ki67-positive brown adipocytes even at Day 1 but not that of SV cells. In addition, the b3-AR antagonist, but not b1-AR antagonist, attenuated the cold exposure-induced increase in the number of Ki67-positive brown adipocytes

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with ki67P elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>1510 publications with ki67P and Brain
Skin Antibodies	>1007 publications with ki67P and Skin
Lung Antibodies	>792 publications with ki67P and Lung
Prostate Antibodies	>545 publications with ki67P and Prostate
Intestine Antibodies	>468 publications with ki67P and Intestine
Kidney Antibodies	>422 publications with ki67P and Kidney
Eye Antibodies	>330 publications with ki67P and Eye
Bone Marrow Antibodies	>298 publications with ki67P and Bone Marrow
Embryonic Tissue Antibodies	>282 publications with ki67P and Embryonic Tissue
Thyroid Antibodies	>265 publications with ki67P and Thyroid