8-Iso Prostaglandin F2alpha, 8-Iso-Pgf2a (8-iso-PGF2a) ELISA Kit for Human

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for 8-iso-PGF2a and Horseradish Peroxidase (HRP) conjugated 8-iso-PGF2a. The competitive inhibition reaction is launched between with HRP labeled 8-iso-PGF2a and unlabeled 8-iso-PGF2a with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of 8-iso-PGF2a in the sample. The color development is stopped and the intensity of the color is measured.

Small volumes of 8-iso-PGF2a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS700957 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 8-iso prostaglandin F2alpha, 8-iso-PGF2a, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 8-iso-PGF2a. The ELISA analytical biochemical technique of the MBS700957 kit is based on 8-iso-PGF2a antibody-8-iso-PGF2a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 8-iso-PGF2a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 8-iso-PGF2a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).