Purine Nucleoside Phosphorylase (PNP) ELISA Kit for Human

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Purine Nucleoside Phosphorylase (also known as PNP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human PNP antibody. PNP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human PNP Antibody is added and binds to PNP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PNP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human PNP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of PNP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS166730 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the purine nucleoside phosphorylase (PNP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PNP. The ELISA analytical biochemical technique of the MBS166730 kit is based on PNP antibody-PNP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PNP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PNP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).