Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1 Alpha (PGC1alpha) ELISA Kit for Human

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human PGC1alpha antibody. PGC1alpha present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PGC1alpha Antibody is added and binds to PGC1alpha in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PGC1alpha antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PGC1alpha. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (also known as PGC1alpha) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Small volumes of PGC1alpha elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS166855 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC1alpha) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PGC1alpha. The ELISA analytical biochemical technique of the MBS166855 kit is based on PGC1alpha antibody-PGC1alpha antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PGC1alpha antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PGC1alpha. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).