Cytochrome P450 3a5 (CYP3A5) ELISA Kit for Human

Name: Human CYP3A5 ELISA Kit; Cytochrome P450 3a5; MyBioSource
Brand: MyBioSource
SKU: MBS166915
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS166915

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CYP3A5 antibody. CYP3A5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CYP3A5… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Cytochrome P450 3A5 (CYP3A5), ELISA Kit

Full Product Name

Human Cytochrome P450 3A5 (CYP3A5) ELISA Kit

Product Gene Name

CYP3A5 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids

Detection Range

10-2000ng/L

Sensitivity

5.14ng/L

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

15,846 Da

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CYP3A5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CYP3A5 purchase

MBS166915 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome P450 3A5 (CYP3A5) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CYP3A5. The ELISA analytical biochemical technique of the MBS166915 kit is based on CYP3A5 antibody-CYP3A5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CYP3A5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CYP3A5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CYP3A5. It may not necessarily be applicable to this product.

NCBI GI #

628601851

NCBI GeneID

1577

NCBI Accession #

NP_001278759.1

UniProt Secondary Accession #

Q53WY8; Q75MV0; Q9HB56; A4D289; B7Z5I7

UniProt Related Accession #

P20815

GenBank Nucleotide #

NM_001291830.1

NCBI Official Full Name

cytochrome P450 3A5 isoform 4

NCBI Official Synonym Full Names

cytochrome P450 family 3 subfamily A member 5

NCBI Official Symbol

CYP3A5

NCBI Official Synonym Symbols

CP35; PCN3; CYPIIIA5; P450PCN3

UniProt Protein Name

Cytochrome P450 3A5

UniProt Synonym Protein Names

CYPIIIA5; Cytochrome P450 HLp2; Cytochrome P450-PCN3

UniProt Gene Name

CYP3A5

NCBI Protein Information

cytochrome P450 3A5

NCBI Summary for CYP3A5

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of this gene have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

UniProt Comments for CYP3A5

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.MiscellaneousChimeric transcripts, characterized by CYP3A43 exon 1 joined at canonical splice sites to distinct sets of CYP3A5 exons, have been detected. All are possibly produced by trans-splicing. The chimeric transcripts exist in 2 different combinations: CYP3A43 exon 1 joined in frame to CYP3A5 exon 11-13 and CYP3A43 exon 1 joined in frame to CYP3A5 exon 12-13. All chimeric transcripts are expressed at very low levels in the liver (PubMed:11726664).

Research Articles on CYP3A5

1. CYP3A5 genetic polymorphism had a significant influence on tacrolimus pharmacological effects in Chinese liver transplantation patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CYP3A5 elisa kit

Reference Product	PubMed Publications
CYP1A2 elisa kit	>1004 publications with CYP3A5 and CYP1A2
CYP2C9 elisa kit	>759 publications with CYP3A5 and CYP2C9
CYP2C19 elisa kit	>721 publications with CYP3A5 and CYP2C19
CYP2C8 elisa kit	>342 publications with CYP3A5 and CYP2C8
UGT1A1 elisa kit	>107 publications with CYP3A5 and UGT1A1
GSTP1 elisa kit	>61 publications with CYP3A5 and GSTP1
UGT2B7 elisa kit	>47 publications with CYP3A5 and UGT2B7
GSTM1 elisa kit	>44 publications with CYP3A5 and GSTM1
GSTT1 elisa kit	>32 publications with CYP3A5 and GSTT1
UGT1A6 elisa kit	>26 publications with CYP3A5 and UGT1A6

Pathways associated with CYP3A5 elisa kit

Products by Pathway	Pathway Diagram
Aflatoxin Activation And Detoxification Pathway antibodies	Aflatoxin Activation And Detoxification Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
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Drug Metabolism - Cytochrome P450 Pathway antibodies	Drug Metabolism - Cytochrome P450 Pathway Diagram
Drug Metabolism - Cytochrome P450 Pathway antibodies	Drug Metabolism - Cytochrome P450 Pathway Diagram
Irinotecan Pathway antibodies	Irinotecan Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram

Diseases associated with CYP3A5 elisa kit

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>439 publications with CYP3A5 and Liver Diseases
Nervous System Diseases Antibodies	>240 publications with CYP3A5 and Nervous System Diseases
Drug-Related Side Effects and Adverse Reactions Antibodies	>161 publications with CYP3A5 and Drug-Related Side Effects and Adverse Reactions
Kidney Diseases Antibodies	>154 publications with CYP3A5 and Kidney Diseases
Mental Disorders Antibodies	>144 publications with CYP3A5 and Mental Disorders
Drug-Induced Liver Injury Antibodies	>131 publications with CYP3A5 and Drug-Induced Liver Injury
Heart Diseases Antibodies	>131 publications with CYP3A5 and Heart Diseases
Inflammation Antibodies	>125 publications with CYP3A5 and Inflammation
Hypertension Antibodies	>114 publications with CYP3A5 and Hypertension
Pain Antibodies	>113 publications with CYP3A5 and Pain

Organs/Tissues associated with CYP3A5 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>4150 publications with CYP3A5 and Liver
Blood Antibodies	>2045 publications with CYP3A5 and Blood
Intestine Antibodies	>747 publications with CYP3A5 and Intestine
Kidney Antibodies	>683 publications with CYP3A5 and Kidney
Lung Antibodies	>207 publications with CYP3A5 and Lung
Brain Antibodies	>181 publications with CYP3A5 and Brain
Prostate Antibodies	>103 publications with CYP3A5 and Prostate
Muscle Antibodies	>79 publications with CYP3A5 and Muscle
Stomach Antibodies	>36 publications with CYP3A5 and Stomach
Placenta Antibodies	>31 publications with CYP3A5 and Placenta