Keratin, Type II Cytoskeletal 1 (KRT1) ELISA Kit for Human

Name: Human KRT1 ELISA Kit; Keratin, Type II Cytoskeletal 1; MyBioSource
Brand: MyBioSource
SKU: MBS166990
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS166990

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human KRT1 antibody. KRT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human KRT1… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Keratin, type II cytoskeletal 1 (KRT1), ELISA Kit

Full Product Name

Human Keratin, type II cytoskeletal 1 (KRT1) ELISA Kit

Product Gene Name

KRT1 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids

Detection Range

0.05-15ng/ml

Sensitivity

0.026ng/ml

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

51,268 Da

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of KRT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT1 purchase

MBS166990 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Keratin, type II cytoskeletal 1 (KRT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KRT1. The ELISA analytical biochemical technique of the MBS166990 kit is based on KRT1 antibody-KRT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KRT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KRT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for KRT1. It may not necessarily be applicable to this product.

NCBI GI #

7717238

NCBI GeneID

3868

NCBI Accession #

AAB30058.2

UniProt Secondary Accession #

P30654; Q16402; Q9UBG8; A8K488

UniProt Related Accession #

P08779

NCBI Official Full Name

keratin, partial

NCBI Official Synonym Full Names

keratin 16

NCBI Official Symbol

KRT16

NCBI Official Synonym Symbols

K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A

UniProt Protein Name

Keratin, type I cytoskeletal 16

UniProt Synonym Protein Names

Cytokeratin-16; CK-16; Keratin-16; K16

Protein Family

Keratin

UniProt Gene Name

KRT16

UniProt Synonym Gene Names

KRT16A; CK-16; K16

NCBI Protein Information

keratin, type I cytoskeletal 16

NCBI Summary for KRT1

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]

UniProt Comments for KRT1

Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.

Research Articles on KRT1

1. results suggest a conditional regulation of KRT16 gene by ATF4 that may be inhibited in normal cells, but engaged during cancer progression. Potential roles of KRT16, FAM129A and HKDC1 genes upregulation in adaptive stress responses and pathologies are discussed

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KRT1 elisa kit

Reference Product	PubMed Publications
FLNA elisa kit	>12 publications with KRT1 and FLNA

Pathways associated with KRT1 elisa kit

Products by Pathway	Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram
Salmonella Infection Pathway antibodies	Salmonella Infection Pathway Diagram
Salmonella Infection Pathway antibodies	Salmonella Infection Pathway Diagram

Organs/Tissues associated with KRT1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>13 publications with KRT1 and Bone
Connective Tissue Antibodies	>4 publications with KRT1 and Connective Tissue
Heart Antibodies	>3 publications with KRT1 and Heart
Muscle Antibodies	>3 publications with KRT1 and Muscle
Brain Antibodies	>3 publications with KRT1 and Brain
Vascular Antibodies	>2 publications with KRT1 and Vascular
Embryonic Tissue Antibodies	>2 publications with KRT1 and Embryonic Tissue
Ear Antibodies	>1 publications with KRT1 and Ear
Kidney Antibodies	>1 publications with KRT1 and Kidney
Umbilical Cord Antibodies	>1 publications with KRT1 and Umbilical Cord