Methylcytosine Dioxygenase TET2 (TET2) ELISA Kit for Human

Name: Human TET2 ELISA Kit; Methylcytosine Dioxygenase TET2; MyBioSource
Brand: MyBioSource
SKU: MBS166992
Price: 425 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS166992

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human TET2 antibody. TET2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TET2… More details

$425 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Methylcytosine dioxygenase TET2 (TET2), ELISA Kit

Full Product Name

Human Methylcytosine dioxygenase TET2 (TET2) ELISA Kit

Product Gene Name

TET2 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids

Detection Range

0.1-40ng/ml

Sensitivity

0.043ng/ml

Preparation and Storage

Store at 2 to 8 degree C for 6 months.

Inter-assay Precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

68,060 Da

3D Structure

ModBase 3D Structure for Q4JK59

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TET2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TET2 purchase

MBS166992 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Methylcytosine dioxygenase TET2 (TET2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TET2. The ELISA analytical biochemical technique of the MBS166992 kit is based on TET2 antibody-TET2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TET2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TET2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TET2. It may not necessarily be applicable to this product.

NCBI GI #

1092878820

NCBI GeneID

214133

NCBI Accession #

NP_001333665.1

UniProt Primary Accession #

Q4JK59

UniProt Secondary Accession #

Q3U5R5; Q3U633; Q3UAI0; Q6ZPN2; Q8K2K3

UniProt Related Accession #

Q4JK59

GenBank Nucleotide #

NM_001346736.1

NCBI Official Full Name

methylcytosine dioxygenase TET2 isoform 2

NCBI Official Synonym Full Names

tet methylcytosine dioxygenase 2

NCBI Official Symbol

Tet2

NCBI Official Synonym Symbols

Ayu17-449; mKIAA1546; E130014J05Rik

UniProt Protein Name

Methylcytosine dioxygenase TET2

UniProt Synonym Protein Names

Protein Ayu17-449

Protein Family

Methylcytosine dioxygenase

UniProt Gene Name

Tet2

UniProt Synonym Gene Names

Kiaa1546

NCBI Protein Information

methylcytosine dioxygenase TET2

UniProt Comments for TET2

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.

Research Articles on TET2

1. TET2 plays an important role in the pathogenesis of diabetic nephropathy by activating TGFbeta1 expression through demethylation of CpG islands in the TGFbeta1 regulatory region

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TET2 elisa kit

Reference Product	PubMed Publications
Asxl1 elisa kit	>280 publications with TET2 and Asxl1
Dnmt3a elisa kit	>275 publications with TET2 and Dnmt3a
Idh1 elisa kit	>203 publications with TET2 and Idh1
Idh2 elisa kit	>147 publications with TET2 and Idh2
Dnmt1 elisa kit	>44 publications with TET2 and Dnmt1
Dnmt3b elisa kit	>34 publications with TET2 and Dnmt3b
Tdg elisa kit	>29 publications with TET2 and Tdg
Foxp3 elisa kit	>12 publications with TET2 and Foxp3
Pou5f1 elisa kit	>7 publications with TET2 and Pou5f1
Cxxc4 elisa kit	>4 publications with TET2 and Cxxc4

Pathways associated with TET2 elisa kit

Products by Pathway	Pathway Diagram
Epigenetic Regulation Of Gene Expression Pathway antibodies	Epigenetic Regulation Of Gene Expression Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
TET1,2,3 And TDG Demethylate DNA Pathway antibodies	TET1,2,3 And TDG Demethylate DNA Pathway Diagram

Diseases associated with TET2 elisa kit

Disease Name	Pubmed Publications
Myelodysplastic Syndromes Antibodies	>205 publications with TET2 and Myelodysplastic Syndromes
Chromosome Aberrations Antibodies	>92 publications with TET2 and Chromosome Aberrations
Cell Transformation, Neoplastic Antibodies	>63 publications with TET2 and Cell Transformation, Neoplastic
Carcinoma Antibodies	>44 publications with TET2 and Carcinoma
Lymphoma, T-Cell, Peripheral Antibodies	>29 publications with TET2 and Lymphoma, T-Cell, Peripheral
Inflammation Antibodies	>27 publications with TET2 and Inflammation
Breast Neoplasms Antibodies	>19 publications with TET2 and Breast Neoplasms
Neoplasms, Experimental Antibodies	>15 publications with TET2 and Neoplasms, Experimental
Fibrosis Antibodies	>15 publications with TET2 and Fibrosis
Immunoblastic Lymphadenopathy Antibodies	>13 publications with TET2 and Immunoblastic Lymphadenopathy

Organs/Tissues associated with TET2 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>309 publications with TET2 and Blood
Bone Marrow Antibodies	>157 publications with TET2 and Bone Marrow
Brain Antibodies	>53 publications with TET2 and Brain
Heart Antibodies	>28 publications with TET2 and Heart
Skin Antibodies	>24 publications with TET2 and Skin
Embryonic Tissue Antibodies	>22 publications with TET2 and Embryonic Tissue
Lung Antibodies	>18 publications with TET2 and Lung
Kidney Antibodies	>10 publications with TET2 and Kidney
Eye Antibodies	>7 publications with TET2 and Eye
Spleen Antibodies	>7 publications with TET2 and Spleen