Bordetella Pertussis IgG (IgG) ELISA Kit for Human

Background: Bordetella pertussis belongs to the genus of Bordetella. It is a small coccoid, gram negative bacillus, which occurs world-wide. Bordetella pertussis is the causative agent of whooping cough, a globally occurring infectious disease that is transmitted from person to person by droplet infection. Children up to the age of 4 years are particularly affected and the mortality in infected infants is high (60 % of deaths occur within the first year after birth). Infections with B. parapertussis result in a less severe form of the disease. The attachment of B. pertussis and B. parapertussis to the ciliated cells in the mucosa of the human respiratory tract is mediated by adhesins. An important adhesion protein and an equally important immunogen is the so-called filamentous haemagglutinin (FHA). Colonisation of the respiratory tract and establishment of infection are facilitated by the synergistic action of several virulence factors. An important virulence factor is pertussis toxin (PT) which mediates multiple biological effects such as leukocytosis, lymphocytosis, mitogenity, and increased sensitivity to histamine. The use of ELISAs for the detection of specific antibodies is the current method of choice for the measurement pf antibodies, e.g. antibodies against the filamentous haemagglutinin (FHA) which are produced in response to contact with Bordetella pertussis and Bordetella parapertussis. As a consequence of this cross reactivity, an immune response to the group-specific antigen provides evidence of contact with Bordetella pathogen.

Principle of the Assay: The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If such antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically.

Intended Uses: The Bordetella pertussis IgA, IgG and IgM ELISA tests are qualitative immunoassays for the detection of human antibodies in serum or plasma directed against Bordetella pertussis and Bordetella parapertussis. These assays are recommended for the sensitive detection of such antibodies in various kinds of samples.

Small volumes of IgG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS490256 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bordetella pertussis IgG, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IgG. The ELISA analytical biochemical technique of the MBS490256 kit is based on IgG antibody-IgG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IgG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IgG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).