Cytokeratin 8 (CK-8) ELISA Kit for Human

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Cytokeratin 8 (also known as CK-8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CK-8 antibody. CK-8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CK-8 Antibody is added and binds to CK-8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CK-8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CK-8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Small volumes of CK-8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS167235 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the cytokeratin 8 (CK-8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CK-8. The ELISA analytical biochemical technique of the MBS167235 kit is based on CK-8 antibody-CK-8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CK-8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CK-8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).