D-Lactic Acid (D-LA) ELISA Kit for Human

Intended Uses: This competitive kit is for the quantification of D-lactic Acid (also known as D-LA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Assay Principle: This kit is a Enzyme-Linked Immunosorbent Assay (ELISA). D-LA standards or samples are added to the wells pre-coated with a monoclonal antibody. Then biotin-conjugated target antigen are added to the wells. The antigens in the standards or samples compete with the biotin-conjugated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of D-LA in the sample. The concentration of D-LA in the sample is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of D-LA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS167715 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the D-lactic Acid (D-LA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing D-LA. The ELISA analytical biochemical technique of the MBS167715 kit is based on D-LA antibody-D-LA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect D-LA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, D-LA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).